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Test Code FLOW Flow Cytometry Immunophenotyping

Useful For

Diagnosis and monitoring of patients with leukemia and lymphoma.

Synonyms/Keywords

Flow Cytometry, Immunophenotyping, Lymphoma / Leukemia Panel

Specimen Requirements

Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No Body fluid Sodium-heparin Green Top Tube (GTT) **See Collection Processing Instructions** 4 mL Dependent on WBC Dependent on WBC
No CSF Sodium-heparin Green Top Tube (GTT); add equal volume of RPMI or HBSS if available **See Collection Processing Instructions** 2 mL Dependent on WBC Dependent on WBC
No Tissue Container/Tube filled with RPMI or HBSS **See Collection Processing Instructions**      
No Blood Sodium-heparin Green Top Tube (GTT) or EDTA Lavender Top Tube (LTT) **See Collection Processing Instructions** 1 mL Dependent on WBC Dependent on WBC
No Bone Marrow Sodium-heparin Green Top Tube (GTT) or EDTA Lavender Top Tube (LTT) **See Collection Processing Instructions**  1mL Dependent on WBC Dependent on WBC

Collection/Processing Instructions

Acceptable specimens include: Fresh Tissue (in sterile RPMI-1640, Hank's Balanced Salt Solution, Cytogenetics Transport Media, or sterile saline); or Body Fluids (submit in sodium heparin).

 

If tissue is large, cut into several smaller pieces. 

 

Specimen must arrive in Flow Cytometry within 36 hours of collection. Notify Flow Cytometry (800-222-5835, ext. 16385) of specimen arrival in lab. Cells in specimen must be viable.

 

Blood and Bone Marrow: Notify Flow Cytometry Laboratory (800-222-5835, ext. 16385) before sending the specimen.  Specimen must arrive within 48 hours of collection.  Cells in the specimen must be viable.  

 

Provide CBC and Differential results and 1-2 (stained or unstained) EDTA slide(s) (preferably from within 1 week of collection of specimen for Flow Cytometry Immunophenotyping).  Markers are selected based upon available clinical history and morphology per pathology protocol. 

 

**For questions regarding other anticoagulants and/or specimen stability, please call 800-222-5835, ext. 16385**

Specimen Stability Information

Specimen Type Temperature Time
Tissue Room temperature 48 - 72 hours
Body fluid Room temperature 48 - 72 hours
CSF Room temperature 48 - 72 hours
Blood Room temperature 48 - 72 hours
Bone Marrow Room temperature 48 - 72 hours

Rejection Criteria

Fixed specimens
Specimens more than 72 hours old
Frozen or refrigerated
**Contact Flow Cytometry for suitability prior to rejection of any specimens.**

Test Information

This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the US Food and Drug Administration.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.

 

Markers are selected based upon available clinical history and morphology per pathology protocol.

Reference Range Information

Performing Location Reference Range
Marshfield Interpretive report

Marshfield Labs Performing Department

Marshfield Labs Flow Cytometry

Performing Information

Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield Monday through Friday, days only 3 days Flow Cytometry

CPT Codes

CPT Modifier
(if needed)		 Quantity Description Comments
88184     Flow cytometry, first marker  
88185     Additional marker, each  
88187     Interpretation, 2 to 8 markers  
88188     Interpretation, 9 to 15 markers  
88189     Interpretation, 16 or more markers  

Outreach CPT Codes

CPT Modifier
(if needed)		 Quantity Description Comments
88184     Flow cytometry, first marker  
88185     Additional marker, each  
88187     Interpretation, 2 to 8 markers  
88188     Interpretation, 9 to 15 markers  
88189     Interpretation, 16 or more markers  

Ordering Applications

Ordering Application Description
Cerner Flow Cytometry Immunophenotyping