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HelpTest Code ABCRNAT COVID 19, Influenza A and B, Respiratory Syncytial Virus (RSV) PCR
Useful For
This is a qualitative molecular test for the detection of nucleic acid of coronavirus 2 (SARS-CoV-2), Influenza virus types A and B and Respiratory Syncytial virus (RSV) in nasopharyngeal swabs from patients presenting with respiratory symptoms. Combination testing is most useful during periods when these viruses are in known circulation in the community. Patients may have mild to severe respiratory illness with symptoms of sore throat, cough, fever, headache, congestion or runny nose, shortness of breath and complications including pneumonia.
Synonyms/Keywords
COVID 19, SARS-CoV-2, Coronavirus, Influenza, RSV, Respiratory
Specimen Requirements
| Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|
| Nasopharyngeal Swab | UTM Universal Viral Transport, M6 Viral Transport, Xpert VTM | One nasophryngeal swab | One nasopharyngeal swab | One nasopharyngeal swab |
Acceptable Body Sites
Nasopharyngeal
Acceptable Specimen Types
Nasopharyngeal swab
Collection/Processing Instructions
Collect a nasopharyngeal specimen using the NP swab. Place swab into the appropriate transport medium and carefully break off the swab shaft against the side of the tube at the score line. Discard the top portion of the swab shaft. Securely tighten the cap of tube.
Transport specimen at 2-8C to Marshfield Labs as soon as possible. If specimen cannot be tested within 72 hours of collection, it should be frozen at -70C or colder until tested.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Nasopharyngeal swab | Refrigerate | ≤ 72 hours |
| Frozen | > 72 hours |
Rejection Criteria
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|---|---|---|---|---|
Test Information
The COVID-19/Influenza/RSV tests used at MCHS have been authorized for use by regulatory authorities including the FDA and CDC. These tests are highly specific (no false-positives) and are considered to be very sensitive, approaching 100%, during symptomatic disease when high titers of viral target are present, including directly before symptoms appear and following symptom resolution for several days. Appropriate collection technique is a critical variable for best test performance.
Negative results do not preclude SARS-CoV-2/Influenza/RSV infection and should not be used as the sole basis for patient management decisions. Negative results must be combined with clinical observations, patient history, and epidemiological information.
Interpretations
Positive Result indicates that the specific virus nucleic acid is detected and suggests infection with the virus.
Negative Result indicates that the specific virus is not detected.
Indeterminate - Result is indeterminate due to a PCR inhibitor present in the specimen. Consider repeat specimen if clinically indicated.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| All Sites | Negative |
Marshfield Labs Performing Department
Marshfield Labs Molecular Pathology
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield Routine | Monday through Sunday | ≤ 24 hours | Nucleic Acid Test (NAT);Real-Time RT-PCR |
| Marshfield STAT | Monday through Sunday | 4 hours | Nucleic Acid Test (NAT);Real-Time RT-PCR |
| Eau Claire | Monday through Sunday | 4 hours | Nucleic Acid Test (NAT);Real-Time RT-PCR |
| Park Falls | Monday through Sunday | 4 hours | Nucleic Acid Test (NAT);Real-Time RT-PCR |
| Ladysmith | Monday through Sunday | 4 hours | Nucleic Acid Test (NAT);Real-Time RT-PCR |
| Lake Hallie STAT* | Monday through Sunday | 4 hours | Nucleic Acid Test (NAT); Real-Time RT-PCR |
| Oakwood STAT* | Monday through Friday | 4 hours | Nucleic Acid Test (NAT); Real-Time RT-PCR. |
| Rice Lake | Monday through Sunday | 4 hours | Nucleic Acid Test (NAT);Real-Time RT-PCR |
| Minocqua | Monday through Sunday | 4 hours | Nucleic Acid Test (NAT);Real-Time RT-PCR |
| Neillsville | Monday through Sunday | 4 hours | Nucleic Acid Test (NAT);Real-Time RT-PCR |
| Wausau STAT* | Monday through Friday | 4 hours | Nucleic Acid Test (NAT); Real-Time RT-PCR |
| Weston | Monday through Sunday | 4 hours | Nucleic Acid Test (NAT);Real-Time RT-PCR |
| Wisconsin Rapids STAT* | Monday through Friday | 4 hours | Nucleic Acid Test (NAT); Real-Time RT-PCR |
| Beaver Dam | Monday through Sunday | 4 hours | Nucleic Acid Test (NAT); Real-Time RT-PCR |
| River Region | Monday through Sunday | 4 hours | Nucleic Acid Test (NAT); Real-Time RT-PCR |
| *STAT only for respiratory season: September-May | |||
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 87637 | 1 | Severe acute respiratorysyndrome coronavirus 2 (SARS-CoV-2) (Coronavirus disease [COVID-19]), influenzavirus types A and B, and respiratory syncytial virus, multiplex amplified probetechnique |
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Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 87637 | 1 | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (Coronavirus disease [COVID-19]), influenza virus types A and B, and respiratory syncytial virus, multiplex amplified probe technique |
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