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HelpTest Code ABSC Antibody Screen
Useful For
Determining the presence of atypical antibodies to red cell antigens in patient plasma or serum. Investigation of immune-mediated hemolytic anemia. Identifying antibody(ies) in prenatal patients to assist in determining the risk for Hemolytic Disease of the Fetus and Newborn (HDFN).
Synonyms/Keywords
Indirect Coombs, Indirect Antiglobulin Test, IAT, Atypical antibody screen
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Whole blood | 6 mL EDTA Pink Top Tube (PTT) |
Two 3 mL EDTA Lavender Top Tubes (LTT)
OR
6 mL Red Top Tube (RTT) |
6 mL | 2.5 mL | Two 0.5 mL EDTA microcollection tubes |
Collection/Processing Instructions
Invert specimen at least 5-6 times in order to facilitate mixing and prevent clotting. Do not separate plasma from red cells.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole blood/serum | Refrigerate | <10 days old |
Rejection Criteria
| Serum separator tubes (SST) |
|---|
| Frozen |
| Hemolyzed |
Test Components
If ordering for transfuion of blood products refer to the following additional Test Codes:
Test Information
An antibody screen is performed to determine the presence of atypical antibodies. An Antibody Screen (ABSC) is the appropriate test to be ordered when requesting an antibody identification. Other Testing Performed: Other tests may or may not be performed, at additional charge, depending on the initial test results. Refer to CPT tables for additional testing codes and description.
Interferences
Fresh serum, fibrin or particulate matter in the patient specimen may cause anomalous results. EDTA plasma may minimize these interferences.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| All Performing Sites | Negative |
Marshfield Labs Performing Department
Marshfield Labs Transfusion Services
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Weston | Monday through Sunday | Less than 2 hours | Solid Phase Red Cell Adherence |
| Park Falls | Monday through Sunday | Less than 2 hours | Column Agglutination Technology |
| Rice Lake | Monday through Sunday | Less than 2 hours | Solid Phase Red Cell Adherence |
| Marshfield | Monday through Sunday | Less than 2 hours | Solid Phase Red Cell Adherence |
| Neillsville | Monday through Sunday | Less than 2 hours | Solid Phase Red Cell Adherence |
| Minocqua | Monday through Sunday | Less than 2 hours | Solid Phase Red Cell Adherence |
| Eau Claire | Monday through Sunday | Less than 2 hours | Solid Phase Red Cell Adherence |
| Beaver Dam | Monday through Sunday | Less than 2 hours | Gel |
| Ladysmith | Monday through Sunday | Less than 2 hours | Gel |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86850 | ABSC | |||
| 86870 | ABID | (if needed) | ||
| 86886 | Antibody Titer | (if needed) |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86850 | ABSC | |||
| 86870 | ABID | (if needed) | ||
| 86886 | Antibody Titer | (if needed) |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Centricity | Antibody Screen |
| Cerner | Antibody Screen |