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Test Code AMY-ISO Amylase Isoenzymes

Useful For

Hyperamylasemia can occur in salivary gland disorders, peritonitis, ruptured ectopic pregnancy, renal failure, acute appendicitis, burns diabetic acidosis, drug addiction and macroamylasemia.  When hyperamylesemia occurs for no apparent reason or other tests do not confirm pancreatitis, macroamylasemia should be considered.  

Synonyms/Keywords

Amylase fractionation, Amylase, Isoenzymes

Specimen Requirements

Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No Serum/Plasma Serum Separator Tube (SST) Red Top Tube    (RTT), Lithium-heparin Plasma Separator (PST) 1 mL 0.5 mL  

Specimen Stability Information

Specimen Type Temperature Time
Serum/Plasma Refrigerate 7 days
Frozen >7 days

Rejection Criteria

Specimens collected with EDTA, oxalate, or sodium citrate preservatives.

Test Components

Total Amylase, pancreatic (p-) amylase, a qualitative screening test for macroamylase

Reference Range Information

Performing Location Reference Range
Marshfield

Total amylase:        

0 - 14 days:       ≤8 U/L

15 days - <3 mo:     ≤17 U/L       

3 mo - <1 yr:     ≤39 U/L                

 ≥1yr:  18 - 69 U/L

Pancreatic amylase: 13 - 53 U/L
Macroamylase: Negative

Marshfield Labs Performing Department

Marshfield Labs Chemistry

Performing Information

     

Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield Monday through Sunday Less than 2 hours CNPG3 Rate, G7 PNP Immunoinhibition, Polyethylene Glycol Precipitation Assays/Beckman AU

CPT Codes

CPT Modifier
(if needed)		 Quantity Description Comments
82150     Amylase, pancreatic  
82150     Macroamylase  

Outreach CPT Codes

CPT Modifier
(if needed)		 Quantity Description Comments
82150     Amylase, pancreatic  
82150     Macroamylase  

Ordering Applications

Ordering Application Description
Cerner Amylase Isoenzymes