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Test Code APLSAB Antiphospholipid Antibodies

Useful For

This semi-quantitative test panel detects IgA, IgG and IgM antibodies to Cardiolipin and Beta-2 Glycoprotein to aid in the diagnosis of primary Antiphospholipid Syndrome (APS) and those secondary to systemic lupus erythematosus (SLE) or SLE-disorders.

Synonyms/Keywords

Anti-Phospholipid Antibodies, Anticardiolipin Antibodies, Antiphospholipid Antibodies, Cardiolipin, Cardiolipin Antibodies, Antiphospholipid syndrome, APS, anti-B2-GP1 antibodies, Beta 2 glycoprotein, Beta-2 glycoprotein, Beta-2 GP1, systemic lupus erythematosus, SLE

Specimen Requirements

Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No Serum Red Top Tube (RTT) Serum Separator Tube (SST) 1 mL 0.5 mL 0.5 mL

Collection/Processing Instructions

Separate serum from cells within 2 hours of collection.

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated 7 days
Frozen >7 days

Rejection Criteria

Gross hemolysis
Gross lipemia
Heat-inactivated samples

Test Information

The APLSAB test utilizes multiplex flow immunoassay, a methodology that greatly resembles traditional EIA, but permits simultaneous detection and identification of many antibodies in a single tube.

 

Antiphospholipid syndrome (APS) is a systemic autoimmune disorder characterized by the venous and/or arterial thrombosis, thrombocytopenia, recurrent fetal loss, and moderate-to-high titers of antiphospholipid (aPL) antibodies, lupus anticoagulant (LA), anti-Cardiolipin (aCL) antibodies, and anti-B2-GP1 antibodies.  A definitive diagnosis of APS requires the presence of at least one clinical manifestation and on positive laboratory result.  Due to the heterogeneous nature of aPL antibodies, combined measurements of ACL and B2GP1 antibodies may provide better sensitivity than either assay alone.

 

Reference Range Information

Performing Location Reference Range
Marshfield

ACL IgA APL U/mL, ACL IgG GPL U/mL, ACL IgM MPL U/mL: 

Negative <20

Positive 20

 

Beta 2 Glycoprotein IgA U/mL, Beta 2 Glycoprotein IgG U/mL, Beta 2 Glycoprotein IgM U/mL: 

Negative <20

Positive 20

Marshfield Labs Performing Department

Marshfield Labs Immunodiagnostics

Performing Information

Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield Monday through Friday, 0700 to 1600 2 to 4 hours Multiplex Flow Immunoassay/BioRad Bioplex 2200

CPT Codes

CPT Modifier
(if needed)		 Quantity Description Comments
86147   3 Cardiolipin Antibodies IgA, IgG, IgM  
86146   3 Beta 2 Glycoprotein 1 Antibodies IgA, IgG, IgM  

Outreach CPT Codes

CPT Modifier
(if needed)		 Quantity Description Comments
86147   3 Cardiolipin Antibodies IgA, IgG, IgM  
86146   3 Beta 2 Glycoprotein 1 Antibodies IgA, IgG, IgM  

Ordering Applications

Ordering Application Description
COM Antiphospholipid Antibodies
Portal Antiphospholipid Antibodies
Cerner Antiphospholipid Antibodies