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HelpTest Code B27 HLA-B27
Useful For
HLA-B27 antigen is strongly associated with ankylosing spondylitis, a chronic inflammatory disease of the axial musculoskeletal system and a few other rheumatic disorders such as Reiter's syndrome, acute anterior uveitis, and inflammatory bowel disease. HLA-B27 testing is routinely used to screen for ankylosing spondylitis since 90% of patients with ankylosing spondylitis have the HLA-B27 surface antigen compared to only 8% of healthy individuals.
Synonyms/Keywords
Flow Cytometry, B27
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Whole blood | EDTA Lavender Top Tube (LTT) or EDTA Pink Top Tube (PTT) | Sodium-heparin Green Top Tube (GTT) ACD Soln. A Yellow Top Tube | 1 mL | 1 mL | 0.5 mL |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole blood | Room temperature | 5 days |
Rejection Criteria
| Refrigerated or frozen |
|---|
| Fixed specimens |
| **Contact Flow Cytometry for suitability prior to rejection of any specimens.** |
Test Information
This test was developed and its performance characteristics determined by Marshfield Labs. It has not been cleared or approved by the US Food and Drug Administration. This test is used for clinical purposes. It should not be regarded as investigational or for research.
Interpretations
If result is Indeterminate/Borderline, reflex confirmatory testing will be ordered by lab and sent to LabCorp (Test code HLA27SO).
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield | Negative |
Marshfield Labs Performing Department
Marshfield Labs Flow Cytometry
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday, Wednesday, and Friday | 4 days | Flow Cytometry |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86812 |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86812 |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | HLA-B27 |