Home
HelpTest Code B27. HLA-B27
Useful For
HLA-B27 antigen is strongly associated with ankylosing spondylitis, a chronic inflammatory disease of the axial musculoskeletal system and a few other rheumatic disorders such as Reiter's syndrome, acute anterior uveitis, and inflammatory bowel disease. HLA-B27 testing is routinely used to screen for ankylosing spondylitis since 90% of patients with ankylosing spondylitis have the HLA-B27 surface antigen compared to only 8% of healthy individuals.
Synonyms/Keywords
Flow Cytometry, B27, B27GL
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
|---|---|---|---|---|---|
| No | Whole blood | EDTA Lavender Top Tube (LTT) or EDTA Pink Top Tube (PTT) | Sodium-heparin Green Top Tube (GTT) ACD Soln. A Yellow Top Tube | 1 mL | 1 mL |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole blood | Room temperature | 5 days |
Rejection Criteria
| Refrigerated or frozen |
|---|
| Fixed specimens |
|
Hemolyzed |
| Clotted |
Interpretations
If result is Indeterminate/Borderline, reflex confirmatory testing will be ordered by lab and sent to LabCorp (Test code HLA27SO).
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield | Negative |
Marshfield Labs Performing Department
Marshfield Labs Flow Cytometry
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday, Wednesday, and Friday | 4 days | Flow Cytometry |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86812 |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86812 |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | HLA-B27. |