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HelpTest Code BLCAGSO Blastomyces Quantitative Antigen, CSF/BAL/Other Body Fluids (316)
Additional Codes
Miravista Diagnostics Test Code: 316
Useful For
Aid in the diagnosis for blastomycosis
Monitor the response to therapy and determine when treatment can be modified or stopped
Monitor Blastomyces antigen levels to help determine relapse of disease
Synonyms/Keywords
Blastomyces Quant Ag, CSF and BF (316)
FL BLCAGSO
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Specimen Minimum Volume (allows for 1 repeat) |
|---|---|---|---|
| No | CSF | Sterile leak-proof container | 0.8 mL |
| No |
Bronchial Lavage Fluid (BAL) | Sterile leak-proof container | 0.5 mL |
| No | Body Fluids | Sterile leak-proof container | 0.5 mL |
Collection/Processing Instructions
Ship to arrive Monday through Friday using a next day delivery service.
Samples may be shipped on dry ice, frozen ice packs or ambient.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| CSF or BAL | Room Temperature | 14 days |
| Refrigerate (preferred) | 14 days | |
| Frozen | 2 months |
Rejection Criteria
- Specimens received cold or room temperature >14 days old
- Non-pipettable specimens, tissue, biopsy, sputum, bronchial brush, tracheal aspirate, FNA, bone marrow, aspirate or stool
- Samples in transport media, fixative or isolator tubes
Test Information
The MVista® Blastomyces Antigen Quantitative EIA can detect and quantify the amount of Blastomyces antigen in patient samples.
During validation testing, the sensitivity was found to be 95.00% and specificity 100% with an assay cutoff of 0.31 ng/mL.
Positive samples may require confirmation which could extend TAT.
Interferences
It is possible that specimens positive for Candida tropicalis, Coccidioides immitis, Coccidioides posadasii, Histoplasma capsulatum, Histoplasma duboisii, Paracoccidioides brasiliensis, Talaromyces marneffei and Aspergillus nidulans may cross-react with the Blastomyces Antigen EIA and test positive.
A variety of interfering substances, as follows, were investigated for each specimen type and none affected the Blastomyces Antigen EIA sensitivity or specificity.
- Urine: glucose, protein, microalbumin, creatine, uric acid, BUN, bilirubin, nitrite, ketones, WBCs, blood/ hemoglobin, antihistamine, ascorbic acid, cough syrup, boric acid, ethyl paraben
- CSF: protein, blood BAL: blood
Interpretations
None Detected: <0.31 ng/mL
Positive: 0.31 ng/mL 20.00 ng/mL
Positive Above the Limit of Quantifications
Results greater than 20.00 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.
Results should be correlated with clinical presentation and history.
Reference Range Information
Reference Interval: None Detected
Reportable Range: 0.31 ng/mL – 20.00 ng/mL
Results above 20.00 ng/mL are reported as 'Positive, Above the Limit of Quantification'
When tested in cultures of 10⁵ – 10⁶ organisms/mL, cross-reactions occurred with Histoplasma spp., Coccidioides spp., Paracoccidioides brasiliensis, Talaromyces marneffei, Aspergillus nidulans, and Candida tropicalis.
Performing Laboratory Name
Miravista Diagnostics
Referral Laboratory Information
| Address | Telephone | Website Link | Marshfield Lab Account # |
|---|---|---|---|
|
4444 Decatur Blvd Suite 300 Indianapolis, IN 46241 |
866-647-2847 | www.miravistalabs.com | MARSH |
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Miravista Diagnostics | Monday through Friday | 1 day | Quantitative sandwich enzyme immunoassay (EIA) |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 87449 |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 87449 |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | Blastomyces Quant Ag, CSF and BF (316) |