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HelpTest Code BORDPCR Bordetella by PCR
Useful For
Bordetella by PCR is the preferred method for the detection of Bordetella pertussis and Bordetella parapertussis, since it is more sensitive than culture, and is less subject to the effects of delayed transport.
Synonyms/Keywords
Bordetella, Pertussis PCR, Whooping Cough, Parapertussis PCR
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Naspharyngeal swab | Universal Transport media- UTM | Universal Viral Transport (UVT, BD) Copan ESwab (Liquid Amies) | 1 swab | 1 swab |
Acceptable Specimen Types
Nasopharyngeal Swabs
Collection/Processing Instructions
Submit one flexible shaft nasopharyngeal swab of Dacron or Rayon in universal transport media. Calcium alginate is NOT acceptable for PCR. Send sample to lab on ice.
For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Swab | Refrigerate (2-8° C) | Up to 7 days |
| Frozen at ≤ -70° C | Up to 5 months |
Rejection Criteria
| Calcium alginate swab |
|---|
| Wooden shaft swab |
| Charcoal swab |
Test Information
BORDPCR is a real-time polymerase chain reaction (PCR) based qualitative in vitro diagnostic test for the direct detection and identification of Bordetella pertussis and Bordetella parapertussis nucleic acid in nasopharyngeal swab (NPS) specimens obtained from individuals suspected of having a respiratory tract infection attributable to B. pertussis or B. parapertussis.
Interpretations
Cross-reactivity with Bordetella holmesii may occur with the Bordetella pertussis polymerase chain reaction (PCR) assay. While the prevalence of B. holmesii is relatively low, it has been associated with pertussis-like symptoms.
Bordetella is a Category 1 reportable disease.
All positive Bordetella results are called to provider.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield |
Negative for Bordetella pertussis by PCR. Negative for Bordetella parapertussis by PCR. |
Marshfield Labs Performing Department
Marshfield Labs Molecular Pathology
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday through Sunday | 6-8 hours | Real Time PCR, IVD |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 87801 |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 87801 |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | Bordetella pertussis by PCR |