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Test Code BPSO Bullous Pemphigoid, BP180 and BP230, IgG Antibodies, Serum

Additional Codes

Mayo Test Code: BPAB


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission container/tube: Plastic vial

Specimen Volume: 1 mL Serum

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Initial screening test in the diagnosis of bullous pemphigoid and its variants

 

Complementing the standard serum test of indirect immunofluorescence utilizing primate esophagus substrate and primate salt-split skin substrate (CIFS / Cutaneous Immunofluorescence Antibodies, IgG and IgG4, Serum)

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

BP 180 and 230, Serum

Specimen Type

Serum Red

Specimen Minimum Volume

Serum 0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 14 days
  Frozen  30 days
  Ambient  14 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

Bullous Pemphigoid 180:

<20 RU/mL (Negative)

≥20 RU/mL (Positive)

 

Bullous Pemphigoid 230:

<20 RU/mL (Negative)

≥20 RU/mL (Positive)

Cautions

As with other diagnostic test procedures, the results obtained with bullous pemphigoid (BP) BP180 and BP230 enzyme-linked immunosorbent assay kit serve only as an aid to diagnosis and should not be interpreted as diagnostic in themselves.

 

Performance of the assay in pediatric patients has not been established.

 

Performance of the assay on other matrices besides serum has not been established.

Day(s) Performed

Varies

Report Available

1 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83516 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BPAB BP 180 and 230, Serum 92671-7

 

Result ID Test Result Name Result LOINC Value
606816 BP 180, S 53842-1
606817 BP 230, S 53843-9

Specimen Retention Time

30 days

Testing Algorithm

For information see Autoimmune Blistering Disease Diagnostic Algorithm.