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HelpTest Code BRUCSO Brucella Abortus Serology (SS02002)
Additional Codes
WSLH Test Code: SS02002
Useful For
To diagnose infection caused by B. abortus, B. melitensis, and B. suis in individuals with possible exposure. Brucellosis in humans has an insidious or abrupt onset, and no pathognomonic symptoms or signs; for these reasons, a serological test may be preferred over isolation of the organism.
Synonyms/Keywords
Brucellosis, Undulant fever, B. abortus, Brucellosis Agglutinins
Specimen Requirements
| Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|
| Serum | Red Top Tube (RTT) | Serum Separator Tube (SST) | 1 mL | 0.5 ml |
Collection/Processing Instructions
Store serum specimen at 2-8° C and transport with cool pack.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated | 7 days |
| Frozen | 365 days |
Rejection Criteria
Hemolyzed, lipemic or heat-inactivated sera are not acceptable
Test Components
Quantitative direct agglutination procedure for the detection of antibody to B. abortus, B. melitensis, and B. suis.
Antibodies to Brucella canis, a rare cause of brucellosis, are not detected by this method.
Test Information
Brucellosis is often related to occupational exposure. Serological results must be critically assessed along with clinical findings and occupational factors before a diagnosis is made.
Interferences
The assay does not detect antibodies to B. canis or other rough types, such as the vaccine strain B. abortus RB51. Serology results should be interpreted in combination with other clinical, laboratory, and epidemiologic findings. Cross-reactions may occur with Francisella tularensis, Vibrio cholerae, Yersinia enterocolitica serotype O:9, Escherichia coli O:15, Afipia clevelandensis, and other antigenically related species.
Interpretations
Titer and interpretation. A single serum titer of >1:160 is suggestive of brucellosis when accompanied by a compatible clinical illness in a patient with potential exposure. A titer of up to 1:80 can be seen in a normal population. Testing of acute and convalescent specimens is recommended for the laboratory diagnosis of brucellosis, with the convalescent specimen collected at least 2 weeks after the acute. In the absence of bacteriologic confirmation, high or a four-fold or greater change in antibody titer is considered a presumptive diagnosis.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Wisconsin State Lab of Hygiene | Negative. Titer and interpretation provided. |
Performing Laboratory Name
Wisconsin State Lab of Hygiene
Referral Laboratory Information
| Address | Telephone | Website Link | Marshfield Lab Account # |
|---|---|---|---|
|
2601 Agriculture Drive Madison, WI 53718 |
800-862-1013 | www.slh.wisc.edu |
815 |
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Wisconsin State Lab of Hygiene | Set up weekly | 2 to 8 days | Microagglutination |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86622 |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86622 |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Centricity | Brucella Ab, IgG & IgM |
| Cerner | Brucella Abortus Serology (WSLH#2) |