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HelpTest Code CARBANAT Carbapenem Resistance Genes, Molecular Detection, PCR
Useful For
Detection of carbapenem-resistance genes from Enterobacterales, Acinetobacter baumannii, and Pseudomonas aeruginosa isolates by nucleic amplified acid test.These bacteria are often resistant to all beta-lactam agents and frequently are co-resistant to multiple classes of other antimicrobial agents, leaving very few treatment options.
Specimen Requirements
| Specimen Type | Preferred Container/Tube | Acceptable Container/Tube |
|---|---|---|
|
Pureculture of aerobic bacterium |
Sealedagar plates |
Agar slant (e.g.,chocolate, tryptic soy) |
Collection/Processing Instructions
Submit pure culture of organism on agar plates to keep turn-around-time to a minimum.Clearly state the name (ID) of the organism submitted for carbapenemase testing. If the identification is not indicated, the organism will be identified, and susceptibility testing will be completed at an additional charge.
Do not send primary plates or plates with mixed cultures. Multiple organisms submitted will be rejected and facility will be asked to resubmit.
Ensure that plates are properly sealed (e.g., tape or parafilm) to prevent contamination and exposure to laboratory personnel.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Agar plate or slant |
Afterincubation, store and transport at room temperature |
5 days |
Rejection Criteria
Isolates submitted that arenot Enterobacterales, P. aeruginosa or Acinetobacter baumannii
Mixed isolation plates
Test Components
blaIMP, blaKPC, blaNDM,blaOXA-48 and blaVIM carbapenem-resistance genes.
Interpretations
Not Detected for all five of the carbapenemase targets: No carbapenemase production.
- Note: A not detected result does not preclude the presence of other carbapenem-resistance mechanisms.
- If there is suspect for any other carbapenemase target the microbiology section will forward the isolate to the state lab upon client request with corresponding sensitivity report.
Detected for any of the five targets: Carbapenemase production.
*Carbapenemase producing organisms are reportable conditions in Wisconsin.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield |
Not Detected |
Marshfield Labs Performing Department
Marshfield Labs Molecular Pathology
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield |
Monday through Sunday |
2 days |
Nucleic Acid Amplification Test |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 87150 |
5 |
Culture, typing; identification by nucleic acid probe, per culture or isolate. |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner |
Carbapenamase Detection by NAT |