Test Code CORD-B Cord Blood Testing
Useful For
Cord Blood Studies are performed on the cord blood of a newborn, when the mother is known Rh(o)D Negative, had an unknown Rh type, or has known significant antibody(ies). The ABO/RH test is performed to determine if a post partum Rh(o)D Negative mother is an Rh Immune Globulin candidate. The DAT is performed to determine if maternal antibody is coating the newborn red cells. When the DAT is positive, elution studies will be performed to determine the maternal antibody(ies) that are coating the cord cells.
Synonyms/Keywords
Direct Antiglobulin Test, Direct Coombs, DAT, Coombs Test, ABO Grouping, Blood Typing, ABO-RH, Type and Rh
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Cord blood | 6 mL EDTA Pink Top Tube (PTT) | 3 mL EDTA Lavender Top Tubes (LTT) | 6 mL | 3 mL | If Cord blood was not collected, Two 0.5 mL microcollection tubes |
Collection/Processing Instructions
Cord blood specimens for blood bank studies must be labeled with baby's first and last name, medical record number, collection date and time, and collector's initials.
DO NOT label the cord blood sample with mother's name or medical record number.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Cord blood | Refrigerate | <48 hours old |
Rejection Criteria
| >48 hrs old |
|---|
| Plasma/Serum must not be separated from the red cells |
| Frozen |
| Hemolyzed |
| Serum separator tube (SST) |
Test Components
ABO/RH, DAT
Test Information
Cord Blood Studies are performed on the cord blood of a newborn, when the mother is known Rh(o)D Negative, had an unknown Rh type, or has known significant antibody(ies). The ABO/RH test is performed to determine if a post partum Rh(o)D Negative mother is an Rh Immune Globulin candidate. The DAT is performed to determine if maternal antibody is coating the newborn red cells. Cord Blood specimens for Transfusion Services studies must be labeled with Baby's first and last name, Medical Record number, Collection Date and Time and collector's initials. Do not label the Cord Blood Sample with Mother's name or medical record number. Order Cord Blood Study in the Hospital computer system. Fill out a Hospital Blood Bank Test Request for the Cord Blood Study with baby's Name, Medical Record Number, Admit number, requesting physician, and mark ABO/RH and DAT in the serological procedures column. If mother is a possible Rh Immune Globulin candidate, fill out a second Hospital Blood Bank Test Request with Mother's Name, Medical Record Number, Admit number, requesting physician, and mark FBSC (Fetal blood screen) in the serological procedures column. Send the properly labeled specimen, Cord Blood order slip, and Hospital blood bank test request(s) to blood bank.
Cord Blood studies include Blood Group and Type (ABO/RH), and Direct Antiglobulin test (DAT). Specimen is tested with polyspecific antiserum. If positive, monospecific IgG and C3 will be tested and charged. When the DAT is positive, elution studies will be performed to determine the maternal antibody(ies) that are coating the cord cells.
Interferences
Whartons jelly may contaminate the specimen
Interpretations
See Critical Value List for list of current critical values
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield | DAT Negative |
Marshfield Labs Performing Department
Marshfield Labs Transfusion Services
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday through Sunday | Less than 2 hours | Serological |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86880 | DAT | |||
| 86900 | ABO | |||
| 86901 | RH |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86880 | DAT | |||
| 86900 | ABO | |||
| 86901 | RH |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | Cord Blood Type and DAT |