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HelpTest Code DAT Direct Antiglobulin Test
Useful For
Demonstrates in vivo coating of red cells with IgG antibody and/or complement
Synonyms/Keywords
Direct Coombs, DAT, Coombs Test, Direct
Direct Antiglobulin Test
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Neonatal Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Whole blood | 6 mL EDTA Pink Top Tube (PTT) |
One 3 mL EDTA Lavender Top Tube (LTT)
Cord blood - EDTA Lavender Top (LTT) |
3 mL | 2.5 mL | One 0.5 mL EDTA microcollection tube |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood | Refrigerate | <48 hours old |
| Cord Blood | Refrigerate | <48 hours old |
Rejection Criteria
| Frozen |
|---|
| Hemolyzed |
| Serum separator tubes (SST) |
| Specimens > 48 hours |
Test Information
Infants >4 months, Pediatrics, and Adults:
Specimen is tested with polyspecific antiserum. If positive, monospecific IgG and C3 will be tested and charged. If positive DAT is from recently transfused patient, an antibody elution will be performed and charged.
Neonates <4 months, including Cord Blood:
Specimen is tested with monospecific IgG. If DAT is positive, an antibody elution may be performed and charged based on maternal antibody history.
Interferences
Some drugs may cause antibody formation, which can lead to a positive DAT, with or without immune hemolysis by one of four mechanisms. Monospecific DAT results may help classify the type of drug mechanism occurring. Drugs known to cause a positive DAT or immune hemolysis can be compared to patients medications to explain unexpected DAT results.
Interpretations
Critical Value: See Critical Value List for list of current critical values
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| All Performing Sites | Negative |
Marshfield Labs Performing Department
Marshfield Labs Transfusion Services
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Beaver Dam | Monday through Sunday | Less than 2 hours | Gel |
| Eau Claire | Monday through Sunday | Less than 2 hours | Serological |
| Marshfield | Monday through Sunday | Less than 2 hours | Serological |
| Rice Lake | Monday through Sunday | Less than 2 hours | Serological |
| Weston | Monday through Sunday | Less than 2 hours | Serological |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86880 |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86880 |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | Direct Antiglobulin Test |