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HelpTest Code DAT* Antibody Elution
Useful For
Used for investigation of positive DAT, and identification of the IgG and/or ABO antibody coating patient cells
Synonyms/Keywords
Elution Study, Eluate
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Neonatal Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Whole blood | 6 mL EDTA Pink Top Tube (PTT) |
One 3 mL EDTA Lavender Top Tube (LTT)
Cord blood - EDTA Lavender Top Tube (LTT) |
3 mL | 2.5 mL | Two 0.5 mL microcollection tubes |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood | Refrigerate | <72 hours old |
| Cord Blood | Refrigerate | <72 hours old |
Rejection Criteria
| Sample >72 hours old will yield weaker results- OK |
|---|
| Serum separator tubes |
| Hemolyzed |
| Frozen |
Test Information
Infants >4 months, Pediatrics, and Adults:
Order antibody elution prerequisite DAT to determine if red cells have been coated in vivo with IgG antibody and/or complement. If positive DAT is from recently transfused patient, an antibody elution will be performed and charged.
Neonates <4 months:
Order antibody elution prerequisite DAT to determine if red cells have been coated with maternal IgG antibody. If DAT is positive, an antibody elution may be performed and charged based on maternal antibody history.
Interferences
Cells with a positive DAT due to complement normally yield an eluate containing no antibody activity.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield | No reference ranges are available |
Marshfield Labs Performing Department
Marshfield Labs Transfusion Services
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday through Sunday | 2-4 hours | Gamma ELU-KIT II or LUI FREEZE method |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86860 |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86860 |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | Direct Antiglobulin Test |