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HelpTest Code EBVCOMP EBV Comprehensive Antibody Panel
Useful For
The serological diagnosis of EBV-related diseases is based upon the detection of antibodies to the EBV VCA, NA and EA antigens (as well as the Paul-Bunnell heterophile antibody). This test should be considered as an aid in the diagnosis of EBV-related chronic and reactivation diseases such as Burkitt's lymphoma, nasopharyngeal carcinoma, and post-transplant lymphoproliferative disease (PTLD).
The detection of Paul-Bunnell heterophile antibody is normally sufficient for the diagnosis of infectious mononucleosis (IM), the most common form of EBV disease; the detection of anti-VCA antibodies alone may also be used in cases of suspected Monospot-negative IM. See Infectious Mononucleosis (IM) and EBV Viral Capsid Antigen IgM & IgG Antibodies (EBVCGM) respectively for information on these tests. Detection of anti-NA and anti-EA is rarely necessary for the diagnosis of IM.
Synonyms/Keywords
Epstein Barr Virus, Early antigen, VCA, nuclear antigen, EBV, Viral Capsid Antigen
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Serum | Red Top Tube (RTT) or Serum Separator Tube (SST) | 1.0 mL | 0.5 mL | 0.2 mL |
Collection/Processing Instructions
Separate serum from cells within 2 hours of collection.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerate | 7 days |
| Frozen | > 7 days |
Rejection Criteria
Hemolyzed specimens
Test Components
Epstein Barr virus (EBV) Viral capsid antigen IgG and IgM; EBV early antigen D IgG; EBV nuclear antigen IgG.
Interpretations
Note that these interpretations are of a general nature, and exceptions exist. Consultation with an infectious diseases specialist should be considered when diagnosing suspected chronic EBV disease.
Primary IM: VCA IgM+ / VCA IgG+ / EA IgG+ / NA IgG-
Late IM: VCA IgM+ / VCA IgG+ / EA IgG+ / NA IgG usually-
Recovery: VCA IgM- / VCA IgG+ / EA IgG usually- / NA IgG+
Latency: VCA IgM- / VCA IgG+ / EA IgG- / NA IgG+
Reactivation: VCA IgM may reappear / VCA IgG+ / EA IgG may reappear / NA IgG+
(+ indicates antibody may or may not be present)
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield | Negative |
Marshfield Labs Performing Department
Marshfield Labs Immunodiagnostics
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday through Friday | 1 day | Multiplex Flow Immunoassay/Bio-Rad BioPlex 2200 |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86665 | 2 | EBV Viral Capsid IgG and IgM | ||
| 86664 | 1 | EBV nuclear antigen-1 | ||
| 86663 | 1 | EBV Early Antigen-D |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86665 | 2 | EBV Viral Capsid IgG and IgM | ||
| 86664 | 1 | EBV nuclear antigen-1 | ||
| 86663 | 1 | EBV Early Antigen-D |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Centricity | EBV Comprehensive Ab Panel |
| Cerner | EBV Comprehensive Antibody Panel |