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Test Code EGIPNAT Extended Gastrointestinal Panel, NAT

Useful For

The extended GI pathogen panel is a multiplex PCR assay for the detection of multiple bacterial, viral and parasite targets specifically:

-Campylobacter species (Campylobacter jejuni/Campylobacter coli/Campylobacter upsaliensis)

-Plesiomonas shigelloides

-Salmonella species

-Vibrio species (Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio cholerae)

-Vibrio cholerae-Yersinia species

-Enteroaggregative Escherichia coli (EAEC)

-Enteropathogenic E coli (EPEC)

-Enterotoxigenic E coli (ETEC)

-Shiga toxin

-E coli O157

-Shigella/Enteroinvasive E coli (EIEC)

-Cryptosporidium species

-Cyclospora cayetanensis

-Entamoeba histolytica

-Giardia

-Adenovirus F 40/41

-Astrovirus

-Norovirus GI/GII

-Rotavirus A

-Sapovirus

 

Acute diarrhea occurring after 3 days of hospitalization is unlikely to be due to bacterial enteropathogens.  Consider a Clostridium difficile toxin nucleic acid assay for C. difficile-associated nosocomial diarrhea. (See Clostridium difficile Toxin Nucleic Acid).

 

Specified positive stool samples are forwarded to the Wisconsin State Lab of Hygiene (WSLH) for surveillance testing. STEC positive samples will be tested to differentiate stx1 and stx2 and subtyped against that most common serogroups in the US.  Salmonella serotyping is also performed. Surveillance data are generated for public health purposes and are not reported to the medical record unless otherwise requested by Provider.

Synonyms/Keywords

Campylobacter species (C. jejuni/coli/upsaliensis), Plesiomonas shigelloides, Vibrio cholera, Enteroaggregative Escherichia coli (EAEC), Enteropathogenic Escherichia coli (EPEC), Enterotoxigenic Escherichia coli (ETEC), Shiga toxin producing E. coli, Escherichia coli O157, Shigella/Enteroinvasive Escherichia coli (EIEC), Cyclospora cayetanensis, Giardia, Astrovirus, Norovirus GI/GII, Rotavirus A, Gastrointestinal Infections, Cryptosporidium species, Entamoeba histolytica, Sapovirus, Salmonella species, Yersinia species, Vibrio species, Adenovirus F 40/41, Gastrointestinal Pathogen Panel PCR, Stool, Feces, Nucleic Acid Test, Enteric Pathogen

Specimen Requirements

Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
 
No Feces Sterile, Leak-proof container Cary Blair 1 gram 1 gram 1 gram

Acceptable Specimen Types

Feces, Liquid or Soft Raw Stool

Collection/Processing Instructions

NOTE - the preferred specimen container is raw stool in a leak-proof container.

 

If submitting in Cary-Blair, remove cap and place approximately 1 gram of the raw stool into the transport medium or a sufficient amount to bring the liquid level up to the "fill to here" line.

Specimen Stability Information

Specimen Type Temperature Time
Feces Refrigerated 24 hours
Feces in Cary Blair filled to the fill line Refrigerated 4 days

Rejection Criteria

Rectal Swabs
Specimens collected in preservatives other than Cary Blair
Feces refrigerated > 24 hours
Feces in Cary Blair preservative > 4 days
Frozen feces
Specimens from patients hospitalized more than 72 hours

Interferences

Any inhibition of amplification will be detected through the use of an internal amplification control.

Interpretations

A negative result should not rule-out infection in patients with a high pretest probability for gastrointestinal infection. The assay does not test for all potential infectious agents of diarrheal disease.


Positive results do not distinguish between a viable or replicating organism and the presence of a nonviable organism or nucleic acid, nor do they exclude the potential for coinfection by organisms not contained within the panel.


Results of the panel are intended to aid in the diagnosis of illness and are meant to be used in conjunction with other clinical and epidemiological findings. This GI panel should not be used as a test of cure.

Reference Range Information

Performing Location Reference Range
Marshfield Negative (for all targets)

Marshfield Labs Performing Department

Marshfield Labs Molecular Pathology

Performing Information

Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield Monday through Friday 24 hours Nucleic Acid Test

CPT Codes

CPT Modifier
(if needed)		 Quantity Description Comments
 
87507        

Outreach CPT Codes

CPT Modifier
(if needed)		 Quantity Description Comments
 
87507        

Ordering Applications

Ordering Application Description
Cerner Extended Gastrointestinal Panel Nucleic Acid Test