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HelpTest Code ENDOMYS Endomysial IgA Antibody
Useful For
The detection of endomysial antibodies is useful as an aid in the diagnosis of Celiac disease (CD) following a seropositive TTG IgA result, primarily in pediatric patients (ref). CD is an immune-mediated disorder of the intestine, caused by an intolerance to gluten proteins found in wheat, rye, or barley. When individuals with CD ingest gluten, it creates an immune mediated toxic reaction that causes damage to the small intestine. Common symptoms include malabsorption of nutrients, diarrhea, weight loss, anemia, bone disease, and a failure to thrive in infants.
Dermatitis Herpetiformis (DH) is the skin manifestation of CD. It is characterized by blistering, intensely itchy skin. The rash is frequently seen on the face, elbows, knees, and buttocks. (JPAN 2012; 54:136-160)
Synonyms/Keywords
ENDOMYSIAL AB, Anti-endomysial Antibodies, Sprue
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Serum | Red Top Tube (RTT) or Serum Separator Tube (SST) | 0.5 mL | 0.3 mL | 0.3 mL |
Collection/Processing Instructions
Separate serum from cells within 2 hours of collection.
Do not freeze-thaw serum more than 1 time.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerate | <2 days |
| Frozen | >2 days |
Rejection Criteria
| Gross hemolysis |
|---|
| Gross lipemia |
Test Information
Endomysial Ab is highly sensitive and specific for celiac disease. Levels decline after the institution of a gluten-free diet. This test is an indirect fluorescent antibody procedure, based on the subjective interpretation of a microscopic pattern using monkey esophagus substrate.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield | Negative <1:10 |
Marshfield Labs Performing Department
Marshfield Labs Immunodiagnostics
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday, Wednesday, Friday | 1 day | Indirect Immunofluorescence (IFA) |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86231 |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86231 |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Centricity | Endomysial IgA Ab |
| Cerner | Endomysial IgA Ab |