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HelpTest Code EVPNAT Enteric Viral Panel by Nucleic Acid Test
Useful For
Detection of Norovirus GI and GII, Rotavirus A, Adenovirus F40/41, Sapovirus (Genogroups I, II, IV, V), Human Astrovirus (hAstro) in patients with gastroenteritis.
Note: Acute diarrhea occurring after 3 days of hospitalization is unlikely to be due to bacterial enteropathogens. Consider a Clostridium difficile toxin nucleic acid assay for C. difficle-associated nosocomial diarrhea instead of stool nucleic acid test. (see Clostridium difficile Toxin Nucleic Acid).
Synonyms/Keywords
Norovirus, Rotavirus, Adenovirus, Sapovirus, Astrovirus, Feces, Nucleic Acid Test, Enteric Pathogen
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Feces | Sterile, Leak-proof container | Cary Blair | 1 gram | 1 gram | 0.5 gram |
Acceptable Specimen Types
Feces, Liquid or Soft Raw Stool
Collection/Processing Instructions
The preferred specimen is fresh stool in a leak-proof container. Transfer approximately 1 gram of stool specimen to a dry, clean container. Avoid contamination with water or urine.
If submitting in Cary-Blair, remove cap and place approximately 1 gram of raw stool into the transport medium or a sufficient amount to bring the liquid level up to the fill to here" line.
For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Feces (Preferred) | Ambient | 48 hours |
| Refrigerated | 120 hours | |
| Feces in Cary Blair filled to the line | Ambient | 48 hours |
| Refrigerated | 120 hours |
Rejection Criteria
| Frozen feces |
| Rectal swabs |
| Feces outside of stability ranges |
| Specimens collected from inpatients after 3 days of hospitalization without laboratory approval |
| Duplicate specimens within 24 hours |
| If test is ordered in conjunction with the Enteric Parasite Panel and the Enteric Bacterial Pathogen Panel, all tests will be canceled and the Extended Gastrointestinal Panel will be ordered instead. |
Test Components
DNA isolation, multiplex real-time polymerase chain reaction amplification and detection.
Interferences
Any inhibition of amplification will be detected through the use of an internal amplification control.
Interpretations
Negative results in individuals with gastroenteritis may be due to infection by pathogens that are not detected by this test or are non-infectious cases. Positive results do not rule out coinfection with other organisms that may also cause gastroenteritis.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield | Negativefor Norovirus, Rotavirus, Adenovirus,Sapovirus, and Astrovirus |
Marshfield Labs Performing Department
Marshfield Labs Molecular Pathology
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday through Friday | 6-8 hours* | Nucleic Acid Test |
| *Testing performed once/day. Sample must be received in the lab by 0600 to be tested same day. |
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CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 87505 |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 87505 |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | Enteric Viral Panel by Nucleic Acid Test |