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Test Code F-VIII Factor Assay-8 (VIII)

Synonyms/Keywords

Factor VIII Activity, AHF, FVIII

Specimen Requirements

Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
*Platelet Poor Plasma 3.2% Citrated Blue Top Tube (BTT), Citrated Blue Top Tube (BTT)   0.75 mL 0.75 mL 0.5 mL

Collection/Processing Instructions

One 0.75 mL citrated Blue Top Tube (BTT) plasma aliquot. (If more than one coagulation test is ordered, a separate aliquot is needed for each test.)

  • Collect in Citrated Blue Top Tube (BTT)
  • Citrate anticoagulant must be adjusted for HCT >55%
  • Tube must be at least 90% full
  • Invert completely 3-4 times (without shaking) to mix

*See Instructions for: Preparation of Platelet Poor Plasma

Specimen Stability Information

Specimen Type Temperature Time
Plasma Frozen 6 months

Rejection Criteria

Clotted or Hemolyzed
Contamination with IV or Hickman Line fluid
Specimens that thaw during storage or transport

Reference Range Information

Performing Location Reference Range
Marshfield <1 year of age: No reference range established for this age range 
>1 year: 60-135% of normal activity

Marshfield Labs Performing Department

Marshfield Labs Hematology

Performing Information

Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield Monday and Thursday Less than 2 hours Siemens CS2500

 

CPT Codes

CPT Modifier
(if needed)
Quantity Description Comments
85240        

Outreach CPT Codes

CPT Modifier
(if needed)
Quantity Description Comments
85240        

Ordering Applications

Ordering Application Description
Centricity Factor-VIII
Cerner Factor VIII Assay
COM Factor Assay-8 (VIII)