Test Code FSKIN Culture, Fungus-Dermal
Synonyms/Keywords
Dermatophyte culture
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Skin, hair, or nail scrapings | Sterile, leakproof container (e.g. Sage container) | ESwab™ or other Amies medium based swab |
Several skin flakes or nail clippings 1 EswabTM or 1 Amies medium based swab |
1-2 nails, several skin flakes |
Collection/Processing Instructions
Specimens should be delivered to the microbiology laboratory as soon as possible. Culture of specimens older than 24 hours may lead to a reduction in recovery of microorganisms.
Do not freeze specimens at -20oC, or in a frost-free freezer.
Specimens should be collected in sterile leakproof containers without preservatives. Swab-collected specimens are not recommended.
Swabs:
- ESwab™: one swab can be collected for Gram stain, aerobic, anaerobic, and fungal cultures.
- Other Amies medium swab types: collect one swab for every culture and stain (including Gram stain) to be ordered.
See ESwab collection video for more information
See Fungus, Direct Wet Prep (KOHFS) for direct stain information.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Swabs (single, dual) in preservative media (Amies) | Room Temperature | ≤ 4 hours |
| Refrigerated | < 24 hours | |
| Eswabs | Room Temperature |
< 48 hours |
| Refrigerated | ||
| All other specimen types | Room Temperature | < 72 hours |
Rejection Criteria
| Dry Swabs |
|---|
| Specimens submitted in formalin or other preservatives |
| Swab specimens collected in preservative media (Amies) >24 hours |
| ESwab™ Specimens >48 hours |
| Leaking specimens |
| Specimens older than 3 days |
Test Information
The fungal load in a clinical specimen may be low. Specimens with less than the recommended volumes may therefore lead to a false negative result.
ESwab™ is a trademark of Copan Diagnostics.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| All Performing Sites | Culture negative for Fungi |
|
See Critical Value List for list of current critical values. |
|
Marshfield Labs Performing Department
Marshfield Labs Microbiology
Performing Information
| Performing Location | Day(s) Test Performed | Methodology/Instrumentation |
|---|---|---|
| Marshfield | Monday through Friday | Conventional culture, microscopic exam with fungal-specific stain, manual or automated identification methods and nucleic acid sequence analysis as needed. |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 87101 | Culture | |||
| 87106 | (Yeast) Organism Identification | as needed | ||
| 87107 | (Mold) Organism Identification | as needed | ||
| 87153 | Identification by nucleic acid sequencing method | as needed |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 87101 | Culture | |||
| 87106 | (Yeast) Organism Identification | as needed | ||
| 87107 | (Mold) Organism Identification | as needed | ||
| 87153 | Identification by nucleic acid sequencing method | as needed |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | Fungus Culture-Dermal |