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Test Code GLU2H Glucose, 2 HR Post-Glucola

Useful For

The primary use of the oral glucose tolerance test is the diagnosis of diabetes mellitus.  The test measures the ability of the individual to remove an added glucose load from the circulation.  Blood samples are obtained at timed intervals after the ingestion of the test glucose load.

Synonyms/Keywords

Glucose, 2 hr post-Glucola, i-Stat 2 hr post-Glucola

Specimen Requirements

Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)

Yes

8 Hours

 Plasma/Serum Lithium-heparin Plasma Separator (PST), Serum Separator Tube (SST) Lithium or Sodium-heparin Green Top (GTT), Red Top (RTT), Gray Top (GYTT) 1 mL 0.3 mL  
i-Stat only = Whole Blood Lithium-heparin Green Top Tube (GTT)   1 mL 200 uL  

Collection/Processing Instructions

Specimens drawn at 120 minutes only following 75 gram (adult) oral glucose test dose. (The glucola must be consumed within a 5 minute interval; sipping water is permitted.) Serum (RTT) or heparinized plasma is also acceptable when removed from cells within one hour of collection.  

 

Patients should be ambulatory and on a high carbohydrate diet for at least 3 days prior to the test. Patient must be fasting for 8 hours prior to start of the tolerance test. Order fasting glucose separately with this test if specified by physician.  

 

i-Stat:  Sample must be tested within 10 minutes of collection.

Specimen Stability Information

Specimen Type Temperature Time
Plasma/Serum Ambient 5 days
Refrigerate 7 days
i-Stat Whole Blood Ambient 10 minutes

Rejection Criteria

i-Stat testing: clotted samples
i-Stat testing: anticoaulants other than lithium heparin

Interpretations

American Diabetes Association Guidelines for the diagnosis of diabetes mellitus in non-pregnant patients (Diabetes Care Jan 2004, S5-S10) are as follows:
Two separate days in which any two of the following events occur:
Fasting glucose ≥100 mg/dL or,
Random glucose ≥200 mg/dL or,
2 hr glucose following glucose load ≥200 mg/dL.

Reference Range Information

Performing Location Reference Range
All Performing Sites See Interpretation

Marshfield Labs Performing Department

Marshfield Labs Chemistry

Performing Information

Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Beaver Dam Monday through Sunday Less than 2 hours Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Chippewa Falls Monday through Friday Less than 2 hours Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Weston Monday through Sunday Less than 2 hours Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Eau Claire Monday through Sunday Less than 2 hours Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Park Falls Monday through Sunday Less than 2 hours Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Ladysmith  Monday through Sunday Less than 2 hours Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Rice Lake Monday through Sunday Less than 2 hours Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Marshfield Monday through Sunday Less than 2 hours Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Minocqua Monday through Sunday Less than 2 hours Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Neillsville Monday through Sunday Less than 2 hours Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Stevens Point Monday through Friday Less than 2 hours Hexokinase-UV/NAD Endpoint Assay/ Beckman AU
Wisconsin Rapids Monday through Friday Less than 2 hours Modified Hexokinase Method/Siemens Dimension

CPT Codes

CPT Modifier
(if needed)		 Quantity Description Comments
82950   1    

Outreach CPT Codes

CPT Modifier
(if needed)		 Quantity Description Comments
82950   1    

Ordering Applications

Ordering Application Description
Cerner Glucose 2 Hour Post Glucola