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HelpTest Code HEPRL Hook Effect Prolactin
Useful For
Determine if hook effect is contributing to a Prolactin reported result.
Synonyms/Keywords
Hook Effect, Prolactin
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Serum | Serum Separator Tube (SST) | Red Top Tube (RTT), Lithium-heparin Plasma Separator Tube (PST) | 0.5 mL | 0.4 mL | 0.3 mL |
Collection/Processing Instructions
Samples collected in a RTT must be removed from the clot wihin 1 hour for storage or transport. Samples collected in SST or PST must be removed from the primary tube wihin 4 hours for transport.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum/Plasma | Refrigerated | 48 hours |
| Frozen | 30 days |
Test Components
Sample will be analyzed undiluted (1:1), as well as at 1:10, 1:100 and 1:1000 dilutions.
Three results will be reported as follows.
HEPRL: Hook Effect Prolactin (Presence or Absence of Hook Effect)
PROLAC: Prolactin (Final Prolactin result reported)
PRLDIL: Hook Effect Final Dilution
Interferences
Human Anti-Murine Antibodies (HAMA) may develop leading to test interference in patients receiving murine-based agents for chemotherapy or tumor-imaging studies.
Interpretations
The undiluted, 1:10, and 1:100 diluted Prolactin results are compared.
ABSENT: If after correction for dilution factor all results are similar, no hook effect is suspected. Undiluted Prolactin result is reported.
PRESENT: If the 1:10 and/or 1:100 dilutions are not similar to the undiluted sample, hook effect is likely. Sample will continue to be diluted until similar results from one dilution to the next are observed. Diluted Prolactin result is reported along with the final dilution performed.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield: Hook Effect Prolactin | ABSENT (no hook effect) |
| Prolactin |
Female (non-pregnant): 2.8 - 29.2 ng/mL (pregnant): 9.7 - 208.5 ng/mL (post-menopausal): 1.8 - 20.3 ng/mL
Male: 2.1-17.7 ng/mL |
Marshfield Labs Performing Department
Marshfield Labs Chemistry
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Sunday through Saturday | 4 hours | Two-site Sandwich Immunoassay using Direct Chemiluminometric Technology/Siemens Centaur |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 84146 | 1 | Prolactin |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 84146 | 1 | Prolactin |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | Hook Effect Prolactin |