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HelpTest Code HIVR HIV-1,2 Ab/Ag by EIA with Confirmation
Useful For
Used for the detection of antibodies to the human Immunodeficiency virus type 1, including Group O, and/or type 2 in serum.
Synonyms/Keywords
HIV-1 Antibody, HIV-2 Antibody, HIV-1 p24 Antigen, HIV-1, HIV-2
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | EDTA Plasma | EDTA Pink Top Tube (PTT) | EDTA Lavender Top Tube (LTT) | 1 mL | 0.5 mL |
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Serum (Screen only, see Note below) | Red Top Tube (RTT) or Serum Separator Tube (SST) | 1 mL | 0.5 mL |
Collection/Processing Instructions
- Centrifugation and refrigerated/frozen storage must occur within 24 hours of collection for either sample type.
- EDTA plasma samples may be centrifuged immediately after collection.
- If serum is inadvertently collected (see note below), complete clot formation should take place before centrifugation.
- After centrifugation, physically separate plasma/serum from the red cells and send aliquot for analysis.
- Specimens must be free of particulate matter including fibrin.
Note: Serum can only be used for initial HIV testing, it cannot be used for Mayo HVDIP confirmation testing of REACTIVE samples.
If REACTIVE, Mayo Clinic Labs will require an additional 1 mL of EDTA plasma.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma / Serum | Ambient | 3 days |
| Refrigerate | 7 days | |
| 30 days |
Rejection Criteria
| Grossly hemolyzed |
|---|
| Grossly icteric |
Test Components
Single qualitative result for reactivity of HIV-1,2 Antibody/Antigen, with automatic reflex to confirmatory testing.
Test Information
The consenting process changed in July 2010; therefore consent form is not required.
This test uses a 3-tier algorithm: HIV Algorithm
Western blot analysis is no longer recommended nor used to confirm HIV EIA antibody-positive results. The 3-tier algorithm described above will yield unambiguous results for HIV status in virtually all cases. Tier 1 (HIV-1/2 Ag/Ab combination immunoassay) will be performed at Marshfield; tier 2 (HIV-1/HIV-2 Ab differentiation immunoassay) will be automatically reflexed and sent to an appropriate testing laboratory; tier 3, the HIV-1 Viral Load assay (test code: HIVPCR) will be recommended in consultation with the ordering provider. Note that the vast majority of samples will yield a definitive result of HIV status by the end of tier 2.
Interpretations
NON-REACTIVE: This screening test found no evidence of HIV-1 or HIV-2 antibodies or HIV-1 p24 Antigen.
REACTIVE: This screening test is REACTIVE for HIV-1 and/or HIV-2 antibodies or HIV-1 p24 Antigen. An HIV-1 and HIV-2 Antibody Confirmation and Differentiation assay is automatically ordered.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield | Expected Value: Non-Reactive |
| Results are reported as "Non-reactive" or "Reactive" |
Performing Laboratory Name
Marshfield Labs
Marshfield Labs Performing Department
Marshfield Labs Chemistry
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday through Sunday | 2 hours | Immunoenzymatic assay/Beckman Coulter DXI 9000 |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 87389 | 1 | HIV-1 Ag(s), with HIV-1 and HIV-2 Abs, single result |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 87389 | 1 | HIV-1 Ag(s), with HIV-1 and HIV-2 Abs, single result |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | HIV 1,2 Antibody/Antigen w/Confirmation (Common) |