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HelpTest Code HPV-ONL Human Papillomavirus (HPV)
Useful For
This test is useful for the detection of Human Papillomavirus (HPV) from the cervical sample associated with a Pap test.
Please note that this testing can only be performed on a specimen that has been deemed to have adequate cellularity through the cytological screening by Pap test. If Pap testing has been performed on the submitted sample at another lab, please submit a copy of the lab report along with the sample.
If Pap testing has not been performed on the sample, please order HPVSO for the send out test that does not require a Pap. If no Pap test result is available, Marshfield Labs will automatically change order to the send out test.
Synonyms/Keywords
HPV, ThinPrepSpecimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | ThinPrep vial | Green Label Aptima Tube | 1.0 mL | 1.0 mL |
Collection/Processing Instructions
Cervical specimens obtained using a spatula / cytobrush collection device and placed in ThinPrep vial containing PreservCyt solution. Transport ThinPrep specimen at room temperature overnight.Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| ThinPrep specimen | Ambient | 30 days |
| Aptima Specimen Transfer tube | Ambient | 60 days |
Rejection Criteria
Insufficient Amount (Less than 1 mL)
Specimen with no accompanying Pap test
Specimen with inadequate cellularity (determined by Pap test)
Test Information
HPV by Transcription-Mediated Amplification (TMA) may be reflexively ordered following a finding of ASCUS on Pap smear, or as clinically indicated. It may also be ordered separately (test code HPV-ONL) if Pap smear was performed on same specimen and cytology report is available indicating adequate cellularity.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield | Reported as Negative, Positive, or Indeterminate for E6/E7 viral messenger RNA (mRNA) from 14 high-risk types (16/18/31/33/35/39/45/51/52/56/58/59/66/68) of human papillomavirus (HPV) in cervical specimens by TMA. |
Marshfield Labs Performing Department
Marshfield Labs Molecular Pathology
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday through Friday | 2-4 days* | Transcription-Mediated Amplification (TMA) |
| *Testing performed once/day. Specimens will be tested for HPV after adequate cellularity is determined through Pap testing screening. | |||
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 87624 |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner |
HPV Only |