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Test Code HSRAT T-Cell Subsets (CD4/CD8 Ratio)

Useful For

For determining percentages and absolute counts of human helper/inducer (CD4) T-lymphocytes, suppressor/cytotoxic (CD8) T-lymphocytes, and T-lymphocytes (CD3).

Synonyms/Keywords

Flow Cytometry, Helper T-Lymphocyte, CD4, T-cell subsets, CD4/CD8 Count, Antibody Monitoring

Specimen Requirements

Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No Whole blood EDTA Lavender Top Tube (LTT) or
EDTA Pink Top Tube (PTT)		   1 mL 1 mL 0.5 mL

Collection/Processing Instructions

A CBC with differential is required within 4 hours of collection of specimen for this test. Submit results with the specimen for this test or include an order for a CBC with differential. 

 

Specimen must arrive in Flow Cytometry within 72 hours of collection. 

Specimen Stability Information

Specimen Type Temperature Time
Whole blood Room temperature 72 hours

Rejection Criteria

Refrigerated or frozen samples

Test Information

Results reported include CD3 (T-cell total); T-cell absolute CD4 and CD8, including ratio.

 

Absolute counts for this test are based on the CBC results provided at the time of testing, including lymphocyte percentage. 

Reference Range Information

Performing Location Reference Range
Marshfield Age appropriate ranges printed on report

Marshfield Labs Performing Department

Marshfield Labs Flow Cytometry

Performing Information

Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield Monday, Wednesday, and Friday 1 day Flow Cytometry

CPT Codes

CPT Modifier
(if needed)		 Quantity Description Comments
86359     T-cells  
86360     Absolute CD4 and CD8  

Outreach CPT Codes

CPT Modifier
(if needed)		 Quantity Description Comments
86359     T-cells  
86360     Absolute CD4 and CD8  

Ordering Applications

Ordering Application Description
Cerner T-Cell Subsets (CD4/CD8 Ratio)