Test Code IDEN Culture, Organism ID, Aerobic Bacteria
Synonyms/Keywords
Organism ID, Aerobic Bacteria
Specimen Requirements
| Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|
| Pure culture of aerobic bacterium | Sealed agar plates |
Agar slant (e.g. chocolate, tryptic soy)
Broth (e.g. tryptic soy, thioglycollate) |
Collection/Processing Instructions
Submit pure culture of organism on agar plates to keep turn-around-time of identification to a minimum.
Organisms submitted on an agar slant or in an acceptable broth media will require subculture prior to identification.
Do not send primary plates or plates with mixed cultures. Multiple organisms submitted will be identified and charged separately. Delay in identification will occur.
Ensure that plates are properly sealed (e.g. tape or parafilm) to prevent contamination and exposure to laboratory personnel.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Agar Plate or Slant | After incubation, store at room temperature | 5 days |
| Broth | Room temperature | 7 days |
Test Information
If susceptibility testing is desired, please indicate on the test request form, including specific antibiotics to be tested. If not indicated, standard panels will be tested. Susceptibility testing is performed at additional charge. Methods used include disk diffusion, minimum inhibitory concentration (MIC) by broth microdilution, or MIC by Etest®.
A listing of the tested and reported antimicrobial agents can be seen at Antimicrobial Susceptibility Panels.
The microbiology laboratory uses selective reporting of susceptibility results in order to promote rational antimicrobial usage. For more information on selective reporting, please contact the microbiology technical manager.
Standardized AST is not available for all bacterial species, in which case an MIC determined by a non-standardized method and without interpretation may be available upon request.
The microbiology laboratory publishes an annual cumulative antibiogram for commonly isolated bacteria, available on the 2024 Combined Inpatient and Outpatient Antibioram Printed copies are also available from the microbiology laboratory.
Etest® is a registered trademark of bioMerieux Inc.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield | Interpretive report |
Marshfield Labs Performing Department
Marshfield Labs Microbiology
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday through Sunday | 3 days | Conventional culture, standard susceptibility testing, manual or automated identification methods |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 87077 | ||||
| 87184 |
KB disc, as needed |
|||
| 87186 | MIC, as needed | |||
| 87153 | Identification by nucleic acid sequencing method, as needed | |||
| 87147 | Sero-grouping, as needed | |||
| 87185 | Enzyme detection, as needed | |||
| 87181 | E-test, gradient strip, as needed |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 87077 | ||||
| 87184 |
KB disc, as needed |
|||
| 87186 | MIC, as needed | |||
| 87153 | Identification by nucleic acid sequencing method, as needed | |||
| 87147 | Sero-grouping, as needed | |||
| 87185 | Enzyme detection, as needed | |||
| 87181 | E-test, gradient strip, as needed |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | Organism ID, Aerobe |