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HelpTest Code IEPIT Protein-Immunotyping (PEP,GAM,IT) Panel
Useful For
Protein electrophoresis is a useful tool in screening or monitoring patients with protein abnormalities such as hypoproteinemia, hepatodegenerative disease, acute and chronic phase reactions, anemia, polyclonal and monoclonal gammopathies and dysproteinemias. Quantitative immunoglobulins are useful in detecting or monitoring of monoclonal gammopathies or immune deficiencies. Immunotyping is useful in determining or ruling out a monoclonal gammopathy.
Synonyms/Keywords
IF Panel (if Immunotyping is required by interpreter), IEP, Immunoelectrophoresis panel - includes protein electrophoresis, immunoglobulins IgG, IgA and IgM and Immunotyping if needed). IT Panel
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Serum | Red Top Tube (RTT) | Serum Separator Tube (SST) | 2 mL | 1 mL | 1 mL |
Collection/Processing Instructions
Separate serum/plasma from cells within 2 hours of collection.
Please make 3 aliquots of serum for this test panel.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum |
Refrigerate (Preferred) | 10 days |
| Frozen (only 1 freeze/thaw cycle) | 30 days |
Rejection Criteria
Plasma
Test Components
Protein Electrophoresis (Total Protein, Albumin, Alpha-1, Alpha-2, Beta, and Gamma Globulins), Immunoglobulin IgG, IgA and IgM. Immunotyping, if required by interpreter (there is an additional charge for Immunotyping).
Test Information
Includes total serum protein, serum protein electrophoresis by Capillary Electrophoresis and quantitative immunoglobulins (IgG, IgA and IgM). Phenotyping of heavy chains and light chains (kappa and lambda) may also be performed by Immunotyping if required for pathologist/PhD interpretation and will be charged separately.
Interpretations
Increased serum immunoglobulin concentrations occur due to polyclonal or oligoclonal immunoglobulin proliferation in hepatic disease (hepatitis, liver cirrhosis), connective tissue diseases, acute and chronic infections, as well as in the cord blood of neonates with intra uterine and perinatal infections. Elevations of IgG, IgA, or IgM may occur in monoclonal gammopathies such as multiple myeloma (IgG, IgA), macroglobulinemia (IgM), primary systemic amyloidosis, monoclonal gammopathy of undetermined significance (MGUS), and related disorders.
Decreased levels are found in patients with primary or secondary immune deficiencies.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield |
Albumin: 3.70-4.90 g/dL Alpha-1 globulin: 0.20-0.40 g/dL Alpha-2 globulin: 0.50-0.90 g/dL Beta-globulin: 0.60- 1.00 g/dL Gamma-globulin: 0.60-1.40 g/dL
Refer to link provided for Protein, Total Reference Ranges:
Refer to link provided for Immunoglobulin Reference Ranges: |
Marshfield Labs Performing Department
Marshfield Labs Special Chemistry
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday through Friday |
6-8 hours If Immunotyping is required - an additional 24 hours should be expected |
Protein Electrophoresis Capillary electrophoresis / Sebia Capillarys 2 protein system Immunoglobulins Turbidimetric method / The Binding Site, Optilite analyzer Immunotyping electrophoresis/Sebia Capillarys 2 Protein System Total serum protein: Biuret Blanked Endpoint Assay/Beckman AU |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 84165-26 | Protein electrophoresis | |||
| 82784 | 3 | Quantitative immunoglobulins (IgG, IgA, IgM) | ||
| 86334 | Immunotyping (if required) |
|
||
| 84155 | Total serum protein |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 84165-26 | Protein electrophoresis | |||
| 82784 | 3 | Quantitative immunoglobulins (IgG, IgA, IgM) | ||
| 86334 | Immunotyping (if required) |
|
||
| 84155 | Total serum protein |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | Protein-Immunotyping (PEP, GAM, IT) Panel |