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HelpTest Code INSULIN Insulin Assay
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| Yes | Serum | Serum Separator Tube (SST) | Red Top Tube (RTT) | 1.0 mL | 0.5 mL | 0.3 mL |
Collection/Processing Instructions
Patient must be fasting a minimum of eight hours before obtaining specimen.
RTT- physically separate plasma or serum from contact with cells as soon as possible or before transport.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Ambient | 8 hours |
| Refrigerated | 1 day | |
| Frozen | >1 day |
Rejection Criteria
Trace hemolysis
Marked Icterus
Test Information
A glucose level should also be ordered on the same specimen. Patients on long-term insulin therapy may have anti-insulin antibodies which will falsely lower insulin results. Insulin assay measures exogenous as well as endogenous insulin; measurement of C-peptide may be helpful in differentiating the insulin source and in assessing the cause of hypoglycemic states.
Note: after 48 hours of refrigeration, insulin loses about 10% of its activity.
Reference Range Information
| Performing Location |
Reference Range |
|---|---|
| Marshfield |
1.9-23 uIU/mL |
Marshfield Labs Performing Department
Marshfield Labs Chemistry
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday through Friday | 2 hours | Immunoenzymatic assay/Beckman Coulter Unicel DXI |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 83525 |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 83525 |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | Insulin Level Total |