Test Code JO1 JO-1 Antibodies
Useful For
A confirmatory test for the evaluation of patients with signs and symptoms of polymyositis and other CTD. Jo-1 antibodies are frequently presented as a speckled pattern by ANA IFA.
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Serum | Red Top Tube (RTT) or Serum Separator Tube (SST) | 1.0 mL | 0.4 mL | 0.3 mL |
Collection/Processing Instructions
Separate serum from cells within 2 hours of collection.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerate | 7 days |
| Frozen | > 7 days |
Rejection Criteria
| Gross lipemia |
|---|
| Gross hemolysis |
| Heat inactivated samples |
Interpretations
A positive result supports the diagnosis of polymyositis.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield |
Negative: <1.0 AI Analytical measuring range is 0.2 -8.0 AI (Antibody Index) |
Marshfield Labs Performing Department
Marshfield Labs Immunodiagnostics
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday through Friday | 1 day | Multiplex Flow Immunoassay/Bio-Rad BioPlex 2200 |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86235 |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86235 |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | JO-1 Antibodies |