Test Code MTRNASO Trichomonas vaginalis, Nucleic Acid Amplification, Varies
Additional Codes
Mayo Test Code: MTRNA
Useful For
Detecting Trichomonas vaginalis in prostatic massage (VBIII) fluid or male urethral swabs
This test is not intended for use in medico-legal applications.
Reporting Name
T.vaginalis, Misc, Amplified RNASpecimen Type
VariesNecessary Information
Specimen source is required.
Specimen Required
This test should only be performed in men.
Submit only 1 of the following specimens:
Specimen Type: Post-prostatic massage fluid (VBIII)
Supplies: Aptima Urine Transport Tube (T582)
Container/Tube: Aptima Urine Specimen Transport Tube
Specimen Volume: 15 to 20 mL
Collection Instructions:
1. Patient should not have urinated for at least 1 hour prior to specimen collection.
2. Patient should void a small amount of urine prior to prostatic massage. Pre-massage urine can be discarded or submitted for other testing as applicable.
3. Patient then ceases voiding and a prostatic massage is performed by the urologist or other health care professional.
4. Collect post-massage fluid into a sterile, plastic, preservative-free container.
5. Transfer 2 mL of post-massage fluid specimen into the Aptima urine specimen transport tube using the disposable pipette provided within 24 hours of collection. The correct volume of fluid has been added when the fluid level is between the black fill lines on the Aptima urine transport tube.
Specimen Type: Urethral (male only)
Supplies: Aptima Unisex Swab Collection Kit (T583)
Container/Tube: Aptima Unisex Swab
Specimen Volume: Swab
Collection Instructions:
1. Urethral specimens must be collected using an Aptima Unisex Swab Collection kit.
2. Patient should not have urinated for at least 1 hour prior to collection.
3. With a rotating movement, insert swab (blue shaft) 2 to 4 cm into urethra.
4. Once inserted, rotate blue swab gently at least 1 full rotation using sufficient pressure to ensure swab comes into contact with all urethral surfaces. Allow blue swab to remain inserted for 2 to 3 seconds.
5. Place blue swab in the Aptima transport tube provided in collection kit.
6. Snap off blue swab at score line so it fits into closed tube.
7. Cap tube securely and label tube with patient's entire name and collection date and time.
Specimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 30 days | APTIMA VIAL |
| Ambient | 30 days | APTIMA VIAL |
Reject Due To
| Transport tubes containing a cleaning swab or more than 1 swab | Reject |
| Multiple sources on single tube | Reject |
Reference Values
Negative
Cautions
This assay is not US Food and Drug Administration approved for prosthetic massage (VBIII) fluid or male urethral samples. However, the performance characteristics of this test have been established by Mayo Clinic in accordance with CLIA-guidelines.
Reliable results are dependent on adequate specimen collection. Because the transport system used for this assay does not permit microscopic assessment of specimen adequacy, training of clinicians in proper specimen collection techniques is necessary.
Therapeutic failure or success cannot be determined with the APTIMA Trichomonas vaginalis assay since nucleic acid may persist following appropriate antimicrobial therapy.
Results from the APTIMA T vaginalis assay should be interpreted in conjunction with other clinical data and symptoms.
A negative result does not preclude a possible infection because results are dependent on adequate specimen collection. Test results may be affected by improper specimen collection, pre-analytical errors, technical errors, or target levels below the assay limit of detection. Furthermore, a negative result does not preclude a possible infection because the presence of Trichomonas tenax or Pentatrichomonas hominis in a specimen may affect the ability to detect T vaginalis RNA.
Assay performance of the APTIMA T vaginalis assay has not been evaluated in the presence of Dientamoeba fragilis.
This report is intended for clinical monitoring or management of patients; it is not intended for use in medico-legal applications.
Day(s) Performed
Monday through Sunday
Report Available
1 to 4 daysSpecimen Retention Time
7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87661
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MTRNA | T.vaginalis, Misc, Amplified RNA | 46154-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SRC6 | SOURCE: | 31208-2 |
| 35034 | T.vaginalis, Misc, amplified RNA | 46154-1 |
Method Name
Transcription-Mediated Amplification
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.