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HelpTest Code NDNA Anti-DNA By Crithidia
Useful For
A confirmatory test for the evaluation of systemic lupus erythematosus. The Crithidia luciliae nDNA test, detects autoantibodies to nDNA antigens. High titers of anti-DNA, a type of anti-nuclear antibody (ANA), are associated with systemic lupus erythematosus (SLE). Titers of DNA autoantibodies may decrease with successful therapy, and increase in acute recurrence of the disease. DNA-anti-DNA immune complexes play a role in the pathogenesis of SLE through the deposit of the complexes in the kidney and other tissues. For this reason the detection and quantitation of DNA autoantibodies is diagnostically and therapeutically helpful in patients suspected, or known to have SLE.
See the Mixed Connective Tissue Disease Panel Algorithm for suggested testing strategy.
Synonyms/Keywords
nDNA (Double stranded) Antibody, NDNA, Anti-native DNA Antibody
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Serum | Red Top Tube (RTT) or Serum Separator Tube (SST) | 0.5 mL | 0.3 mL | 0.3 mL |
Collection/Processing Instructions
Separate serum from cells within 2 hours of collection.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerate | <2 days |
| Frozen | >2 days |
Rejection Criteria
Gross hemolysis
Test Information
Substrate is Crithidia lucillae. Positives will be titered.
Interpretations
A positive result supports the diagnosis of systemic lupus erythematosus.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield | Negative (<1:10) |
Marshfield Labs Performing Department
Marshfield Labs Immunodiagnostics
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday, Wednesday, Friday | 1 day | Indirect Immunofluorescence (IFA) |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86225 | 1 |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86225 | 1 |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | Anti-DNA by Crithidia |