Test Code NEO ABORH Neonatal ABO/RH
Useful For
Samples from infants less than 4 months old will not regularly contain expected Anti-A or Anti-B in serum; therefore, only RBC ABO typing is required. Rh-positive and Rh-negative phenotypes are determined by the presence or absence of D antigens on RBCs. When Rh typing is performed for newborns of Rh-negative mothers (to determine maternal RhIG candidacy) the method for Rh typing must be capable of detecting weak expression of D; therefore, Weak D testing may also be performed. When fetomaternal ABO incompatibility exists, potential for ABO Hemolytic Disease of the Fetus and Newborn (HDFN) exists.
Synonyms/Keywords
ABO/RH, Type and Rh, ABO/RH Typing, BB Blood Type, ABO & RH
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Whole Blood | EDTA Lavender Top Tube (LTT) | whole blood microcollection tubes (EDTA) | 3 mL | 1 mL | Two 0.5 mL tubes |
Acceptable Specimen Types
Whole Blood
Collection/Processing Instructions
If ordering test for transfusion of blood products, refer to Neonatal Transfusion
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood | Refrigerate | <10 days old |
Rejection Criteria
Frozen, Hemolyzed, Serum Separator Tubes (SST), Specimens > 10 days old
Test Components
ABO, RH
Test Information
ABO blood types are determined by the presence or absence of A and/or B antigens on RBCs, and by the presence or absence of Anti-A and/or Anti-B in the serum. Neonates are defined as infants from birth to less than 4 months of age.
Interpretations
ABO Typing: A, B, AB, O
Rh Typing: Rh positive / Rh negative
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| All Performing Sites | No reference ranges are available |
Marshfield Labs Performing Department
Marshfield Labs Transfusion Services
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Beaver Dam | Monday through Sunday | Less than 2 hours | Gel |
| Eau Claire | Monday through Sunday | Less than 2 hours | Serological |
| Ladysmith | Monday through Sunday | Less than 2 hours | Gel |
| Marshfield | Monday through Sunday | Less than 2 hours | Serological |
| Minocqua | Monday through Sunday | Less than 2 hours | Serological |
| Neillsville | Monday through Sunday | Less than 2 hours | Gel |
| Park Falls | Monday through Sunday | Less than 2 hours | Serological |
| Rice Lake | Monday through Sunday | Less than 2 hours | Serological |
| Weston | Monday through Sunday | Less than 2 hours | Serological |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86900 | 1 | ABO | ||
| 86901 | 1 | Rh |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86900 | 1 | ABO | ||
| 86901 | 1 | Rh |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | Neonatal Direct Antiglobulin Testing |