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HelpTest Code NEO DAT Neonatal Direct Antiglobulin Testing
Useful For
When fetomaternal ABO incompatibility exists, potential for ABO Hemolytic Disease of the Fetus and Newborn (HDFN) exists. This test may be useful in investigation of suspected HDFN. RBCs obtained directly from the patient are washed free of unbound globulins and tested against monospecific Antihuman globulin (AHG) reagents (Anti-IgG). Agglutination by a monospecific reagent suggest that the RBCs are coated with the corresponding globulin.
Synonyms/Keywords
Direct Coombs, DAT, Coombs Test, Direct Antiglobulin Test
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No |
Whole Blood |
EDTA Lavender Top Tube (LTT) |
whole blood microcollection tubes (EDTA) |
3 mL |
1 mL |
Two 0.5 mL tubes |
Acceptable Specimen Types
Whole Blood
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood |
Refrigerate |
<48 hours old |
Rejection Criteria
Frozen, Hemolyzed, Serum Separator Tubes (SST), Specimens >48 hours old
Test Components
DAT IgG
Test Information
Direct antiglobulin testing is used in the investigation of immune-mediated RBC destruction when IgG may be bound to RBCs in vivo. Neonates are defined as infants from birth to less than 4 months of age.
Interferences
Some drugs may cause antibody formation, which can lead to a positive DAT, with or without immune hemolysis by one of four mechanisms. Monospecific DAT results may help classify the type of drug mechanism occurring. Drugs known to cause a positive DAT or immune hemolysis can be compared to patients medications to explain unexpected DAT results.
Interpretations
Neonates <4 months: Specimen is tested with monospecific IgG. The DAT is performed to determine if maternal antibody is coating the newborn red cells. If DAT is positive, an antibody elution may be performed and charged based on maternal antibody history.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| All Performing Sites |
Negative |
Marshfield Labs Performing Department
Marshfield Labs Transfusion Services
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Beaver Dam |
Monday through Sunday |
Less than 2 hours |
Gel |
| Eau Claire |
Monday through Sunday |
Less than 2 hours |
Serological |
| Marshfield |
Monday through Sunday |
Less than 2 hours |
Serological |
| Rice Lake |
Monday through Sunday |
Less than 2 hours |
Serological |
| Weston |
Monday through Sunday |
Less than 2 hours |
Serological |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86880 |
1 |
DAT IgG |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86880 |
1 |
DAT IgG |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner |
Neonatal ABO/RH |