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HelpTest Code PBORBSO Lyme Disease, Molecular Detection, PCR, Blood
Additional Codes
Mayo Test Code: PBORB
Reporting Name
Lyme Disease PCR, BUseful For
Supporting the diagnosis of Lyme disease in conjunction with serologic testing
This test should not be used to screen asymptomatic patients.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Whole Blood EDTAOrdering Guidance
This assay does not detect Borrelia miyamotoi. If infection with this organism is suspected, order BMIPB / Borrelia miyamotoi Detection, PCR, Blood or BMIYC / Borrelia miyamotoi Detection, PCR, Spinal Fluid.
Specimen Required
Container/Tube: Lavender top (EDTA)
Specimen Volume: 1 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Specimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood EDTA | Refrigerated (preferred) | 7 days |
| Frozen | 7 days |
Reference Values
Negative
Reference values apply to all ages.
Day(s) Performed
Monday through Sunday
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87476
87798 x 2
87801 (if appropriate for government payers)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PBORB | Lyme Disease PCR, B | 90892-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 56080 | B. burgdorferi PCR, B | 94247-4 |
| 38290 | B. mayonii PCR, B | 94248-2 |
| 38291 | B. garinii/B. afzelii PCR, B | 94249-0 |
| 38340 | Lyme Disease PCR Comment | 59464-8 |
Cautions
Serologic tests are recommended for diagnosis of Lyme disease. Polymerase chain reaction (PCR) may play an adjunctive role but may not detect Borrelia burgdorferi DNA from blood in cases of active or chronic disease. The presence of inhibitory substances may also cause a false-negative result. PCR test results should be used as an aid in diagnosis and not considered diagnostic by themselves. These results should be correlated with serologic and epidemiologic data and clinical presentation of the patient.
Concurrent infections with multiple tick-borne pathogens, including Ehrlichia muris eauclairensis, Anaplasma phagocytophilum, Babesia microti, and Borrelia miyamotoi (a relapsing-fever Borrelia) have been reported in the United States, and consideration should be given to testing for other pathogens, if clinically indicated.
This assay detects most members of the B burgdorferi sensu lato complex (Bbsl), including Borrelia andersoni, Borrelia americana, and Borrelia bissettii, which have been rarely detected in humans. Detection of DNA from these organisms would be reported as an atypical result and prompt additional laboratory testing to further identify the DNA present. The sensitivity of this assay for detecting these organisms has not been determined.
This assay also detects some members of the Bbsl complex that are not considered to be human pathogens but may be found in ticks and other animals. Therefore, this assay should not be used to test nonhuman specimens.
Report Available
1 to 4 daysSpecimen Retention Time
1 weekReject Due To
| Gross hemolysis | OK |
Method Name
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Testing Algorithm
For more information see Acute Tickborne Disease Testing Algorithm.
Special Instructions
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.