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Test Code RETIC Reticulocyte Count

Useful For

Assessing erythropoetic bone marrow activity in anemia.

Synonyms/Keywords

Retic Count

Specimen Requirements

Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No Whole Blood EDTA Lavender Top Tube (LTT) EDTA Pink Top Tube (PTT) 2 mL One lavender top micro collection tube or 1.0 mL in 3 mL lavender One lavender top micro collection tube with 0.5 mL

Collection/Processing Instructions

Invert specimen at least 5-6 times in order to facilitate mixing and prevent coagulation.  

 

Prepare blood smear as soon as possible, preferably within two hours of collection, up to 24 hours, labeled with computer generated label or use pencil to label with patients full name and date. Store slides at room temperature.

Specimen Stability Information

Specimen Type Temperature Time
Whole blood Ambient <24 hours
Refrigerated <72 hours
Refrigerated >72 hours may be reported with additional comments

Rejection Criteria

Clotted
Grossly Hemolyzed
Diluted with IV or tissue fluid
Frozen

Test Information

Normal ranges were established at Marshfield Labs using non-parametric analysis (95% limits) of results on 100 patient specimens. Reticulocytes are measured using flow cytometry technology and supravital staining with new methylene blue. A computer algorithm converts light scatter and particle count into Reticulocyte % and absolute #. Immature Reticulocyte Fraction (IRF) also reported.

Reference Range Information

Performing Location Reference Range
Marshfield Reticulocyte %: 0.50-1.77%
Reticulocyte #: 22.7-84.5 x10^3/uL
                 IRF: 20.0-43.0%

Marshfield Labs Performing Department

Marshfield Labs Hematology

Performing Information

Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Weston Monday through Sunday Less than 2 hours Electrical Impediance/Beckman Coulter DXH600
Marshfield Monday through Sunday Less than 2 hours Unicel Beckman Coulter DxH800

CPT Codes

CPT Modifier
(if needed)
Quantity Description Comments
85046        

Outreach CPT Codes

CPT Modifier
(if needed)
Quantity Description Comments
85046        

Ordering Applications

Ordering Application Description
Centricity Reticulocyte Count (Retic)
Cerner Reticulocyte Count