Test Code RHIG RH-Immune Globulin
Useful For
Prevention of sensitization and the development of Anti-D by the D(Rh) negative patient
Synonyms/Keywords
RHo-Gam, RhIg
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Whole blood | 6 mL EDTA Pink Top Tube (PTT) |
Two 3 mL EDTA Lavender Top Tubes (LTT) | 3 mL | 2.5 mL | Two 0.5 mL microcollection tubes |
Collection/Processing Instructions
28 Weeks Gestation, First or Second Trimester (bleed, abortion, miscarriages, ectopic pregnancy): ABSC
ABO/RH if no record of ABO/Rh in Transfusion Service computer system
Third Trimester (abortion, miscarriage, ectopic pregnancy, vaginal bleed, fetal death): ABSC, FBSC
ABO/RH if no record of ABO/Rh in Transfusion Service computer system
Postpartum: FBSC (drawn > 1 hour after delivery)
ABO/RH and ABSC if no current record of these tests in Transfusion Service computer system
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole blood | Refrigerate | Perform testing within 48 hours of collection |
Rejection Criteria
| Serum separator tubes (SST) |
|---|
| Frozen samples |
| Hemolyzed samples |
Test Components
ABO/RH, ABSC, FBSC
Test Information
RhIg is a highly concentrated solution of IgG anti-D derived from pooled human plasma and contains about 300mg of anti-D IgG globulin. The administration of RhIg to a D(Rh) negative patient will prevent sensitization and the development of Anti-D by the patient. Send a separate Blood Bank request form for "Rh Immune Globulin".
If FBSC (Fetal Bleed Screen) is positive, the physician must be notified that a Kleihauer-Betke test is required. The Kleihauer-Betke test will determine the number of vials of RhIg needed.
Refer to Fetal Bleed Screen Test procedure
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield | No reference ranges are available |
Marshfield Labs Performing Department
Marshfield Labs Transfusion Services
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday through Sunday | Less than 2 hours | Serological |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86999 |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86999 |