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Test Code ROTASO Rotavirus Antigen, Feces

Additional Codes

Mayo test code: ROTA

 

Reporting Name

Rotavirus Ag, F

Useful For

Investigation of patients with diarrhea, particularly infants, the elderly, and immunocompromised patients

 

Investigation of nosocomial diarrhea

Testing Algorithm

For information on other diagnostic tests that may be useful in the evaluation of a patient with diarrhea, see Laboratory Testing for Infectious Causes of Diarrhea.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Fecal


Specimen Required


Specimen Type: Fresh unpreserved feces

Supplies: Stool Collection Kit, Random (T635)

Container/Tube:

Preferred: Sterile fecal container

Acceptable: Swab

Specimen Volume: 5 to 10 grams

Collection Instructions: Place specimen in a tightly sealed plastic bag.


Specimen Minimum Volume

1 gram

Specimen Stability Information

Specimen Type Temperature Time
Fecal Frozen (preferred) 7 days
  Refrigerated  72 hours

Reference Values

Negative

Day(s) Performed

Monday through Friday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87425

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ROTA Rotavirus Ag, F 5880-0

 

Result ID Test Result Name Result LOINC Value
24082 Rotavirus Ag, F 5880-0

Cautions

Fecal specimens should be collected as soon after onset of symptoms as possible.

 

Do not collect specimens in containers having media, preservatives, animal serum, or detergent as any of these may interfere with the assay.

 

A positive result does not preclude the presence of other pathogenic organisms. While the relationship between rotavirus and gastroenteritis is well established, coinfection with bacterial or parasitic pathogens is possible. If suspected, testing for other enteric pathogens should be performed in parallel with the rotavirus antigen test.

 

Results of the rotavirus antigen assay must be interpreted with caution. A negative result does not exclude the possibility of rotavirus infection, as too small a quantity of virus or inadequate or improper sampling may cause a false-negative result.

Report Available

1 to 3 days

Specimen Retention Time

7 days

Reject Due To

Grossly bloody (containing no visible feces)
Very mucoid feces
Feces received in any preservative (example: Ecofix, formalin, PVA, C and S media)		 Reject

Method Name

Enzyme Immunoassay (EIA)

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Microbiology Test Request (T244)

-Gastroenterology and Hepatology Test Request (T728)