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Test Code RPHIV OB/ER Only HIV 1/2 Rapid Test

Important Note

For internal ordering only, and restricted to select sites. See details below. Not available to outreach clients.

Useful For

Identifying HIV infection in O.B. and E.R. patients with unknown HIV serostatus at time of delivery and at time of presentation in E.R.


Restricted Use: This test is only orderable and valid for Marshfield Medical Center (01), Weston (Hospital) (71), and Rice Lake (64).

Synonyms/Keywords

HIV

Specimen Requirements

Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No Plasma EDTA Pink Top Tube (PTT) EDTA Lavender Top Tube (LTT) 1 mL 0.5 mL  

Collection/Processing Instructions

Centrifugation and refrigerated/frozen storage must occur within 24 hours of collection for either sample type.   

   

EDTA plasma samples may be centrifuged immediately after collection.

 

If serum is inadvertently collected (see note below), complete clot formation should take place before centrifugation.      

 

After centrifugation, physically separate plasma/serum from the red cells and send aliquot for analysis.        

 

Specimens must be free of particulate matter including fibrin.    

          

Note: Serum can only be used for initial HIV testing, it cannot be used for Mayo HVDIP confirmation             testing of REACTIVE samples.          

 

Specimens are stable for 24 hours at room temperature, this includes pre- & post- centrifugation time, separated or unseparated from red cells.        

 

Store plasma/serum specimens at 28oC if not tested within 24 hours of collection.  Separated specimens are stable for 14 days at 28oC.        

 

For longer storage, plasma/serum may be frozen for 8 months at -20oC or colder.

 

Samples may undergo up to 5 freeze/thaw cycles.   

     

Frozen samples must be completely thawed, thoroughly mixed, and centrifuged before analysis.   

 

For U-have testing, specimens are stable in primary tubes up to 14 days at 28oC (this includes plasma stored on packed red cells, serum stored on the clot, and specimens processed and stored in gel barrier blood collection tubes).              

 

If REACTIVE, Mayo Medical Labs will require an additional 1 mL of EDTA plasma.

Specimen Stability Information

Specimen Type Temperature Time
Plasma Ambient 24 hours
Refrigerate 14 days
Frozen (-20 oC)  8 months

Rejection Criteria

Grossly icteric specimens are uninterpretable due to high background
Specimens containing precipitate
Cadaver specimens
Heat-inactivated specimens
Body fluids other than serum
Samples stored at room temperature for longer than 24 hours, this includes pre- and post- centrifugation time.
Samples stored for longer than 14 days at 28oC.
Specimens with obvious microbial contamination.

Test Information

This test is for use by OB and ER providers only, for patients presenting that have no known HIV status, and the risk of exposure to blood is significant during the patient encounter.

 

Do not order this test for the routine determination of HIV serostatus: Use HIV-1,2 Antibody EIA with Confirm Eval.

 

Do not order for identifying HIV infection in source patient in a known exposure incident with a healthcare worker: Use HIV-1/2 Exposure Screen.

Interpretations

Western blot analysis is no longer recommended for confirmation of HIV EIA antibody-positive results.

 

Confirmation of rapid HIV 1,2 antibody testing will be performed at Mayo Medical Labs.

 

This test algorithm closely follows the current CDC recommendations for HIV diagnosis. (See Centers for Disease Control and Prevention. Detection of Acute HIV Infection in Two Evaluations of a New HIV Diagnostic Testing Algorithm United States, 20112013. MMWR 2013;62:489-494.)

Reference Range Information

Performing Location Reference Range
All sites Non-Reactive

Marshfield Labs Performing Department

Marshfield Labs Chemistry

Performing Information

Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Diagnostic & Treatment Center Monday through Sunday 2 hours Lateral flow immunoassay/Trinity Biotech Uni-Gold Recombigen HIV-1/2 Rapid Test
Eau Claire Monday through Sunday 2 hours Lateral flow immunoassay/Trinity Biotech Uni-Gold Recombigen HIV-1/2 Rapid Test
Lakeview Medical Center Monday through Sunday 2 hours Lateral flow immunoassay/Trinity Biotech Uni-Gold Recombigen HIV-1/2 Rapid Test
Marshfield Monday through Sunday 2 hours Sandwich Chemiluminometric IA/Siemens Centaur
Minocqua Monday through Sunday 2 hours Lateral flow immunoassay/Trinity Biotech Uni-Gold Recombigen HIV-1/2 Rapid Test

CPT Codes

CPT Modifier
(if needed)		 Quantity Description Comments
87389   1 HIV-1 Ag(s), with HIV-1 and HIV-2 Abs single result For testing performed at Marshfield
86703 92 1 HIV-1 and HIV-2 Abs single result For testing performed at Regional Center Labs

Outreach CPT Codes

CPT Modifier
(if needed)		 Quantity Description Comments
87389   1 HIV-1 Ag(s), with HIV-1 and HIV-2 Abs single result For testing performed at Marshfield
86703 92 1 HIV-1 and HIV-2 Abs single result For testing performed at Regional Center Labs

Ordering Applications

Ordering Application Description
Centricity OB/ER Only HIV 1/2 Rapid Test