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Test Code RPMPMSO Mycoplasma (Mycoplasmoides) pneumoniae Macrolide (Azithromycin) Resistance Prediction, Molecular Detection, PCR, Varies

Additional Codes

Mayo Test Code: RPMPM


Ordering Guidance


This test should only be ordered on specimens that have tested positive for Mycoplasma (Mycoplasmoides) pneumoniae. This assay predicts M pneumoniae macrolide (Azithromycin) resistance only.

 

For detection of M pneumoniae prior to macrolide resistance testing , order MPRP / Mycoplasma (Mycoplasmoides) pneumoniae with Macrolide Resistance Reflex, Molecular Detection, PCR, Varies.



Necessary Information


Specimen source is required; include the specific anatomic source.



Specimen Required


The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasma (Mycoplasmoides) pneumoniae DNA is unlikely.

 

Submit only 1 of the following specimens:

 

Specimen Type: Swab

Supplies:

-Culturette (BBL Culture Swab) (T092)

-BD E-swab (T853)

-Culture Swab-Liquid Stuarts/Single Swab (NP Swab) (T515)

-M4-RT (T605)

Sources: Throat, nasal, or nasopharyngeal

Container/Tube:

Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)

Acceptable: Culture transport swab (Stuart's media) or place swab in M4, M4-RT, M5, M6, universal transport media, or ESwab

Specimen Volume: Swab

Collection Instructions:

1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.

2. Place swab back into swab cylinder.

 

Specimen Type: Fluid

Sources: Pleural, pericardial, cerebrospinal

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

 

Specimen Type: Respiratory

Sources: Bronchial washing, bronchoalveolar lavage, tracheal secretions, sputum

Container/Tube: Sterile container

Specimen Volume: 1 mL


Useful For

Predicting macrolide susceptibility in Mycoplasma (Mycoplasmoides) pneumoniae

Method Name

Rapid Polymerase Chain Reaction (PCR) using Light Cycler and Fluorescent Resonance Energy Transfer (FRET)

Reporting Name

M. pneumoniae Macrolide Resist PCR

Specimen Type

Varies

Specimen Minimum Volume

Respiratory: 0.5 mL
Other specimen types: See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Cotton or calcium alginate-tipped swab, wooden shaft swab, transport swab containing gel or charcoal
Port-a-Cul tube
Anaerobic fluid vials
Dry swab (no pledget or sponge)
Respiratory fluid specimens placed in viral transport medium (VTM) or placed on a swab and then into VTM (M4-RT, M4, or M5)
Body fluid specimens placed in viral transport medium (VTM) or placed on a swab and then in VTM (M4-RT, M4, or M5)		 Reject

Reference Values

Not Predicted

Cautions

This assay should only be used for testing of respiratory tract specimens (throat swabs, nasopharyngeal swabs, tracheal secretions, sputum, and bronchoalveolar lavage fluid) and pleural/chest fluid, pericardial fluid, and cerebrospinal fluid that has already tested positive for Mycoplasma (Mycoplasmoides)pneumoniae using a nucleic acid amplification test.

 

Test results should be used as an aid in the diagnosis. The single assay should not be used as the only criterion to form a treatment decision; results of this test should be correlated with clinical presentation and results of other laboratory tests. A negative result does not negate the presence of the organism or active disease.

 

Rarely encountered Mycoplasma species may be detected with this assay when present at high concentrations, however this assay is intended to be used as reflex for previously identified M pneumoniae positive samples. Therefore, cross reactivity with other Mycoplasma species is not a major concern.

 

This assay examines the two most common 23S ribosomal RNA single point variants associated with high-level macrolide resistance. Other mechanisms of macrolide resistance are not assessed nor are mechanisms of resistance to non-macrolide antimicrobial agents.

Day(s) Performed

Monday through Sunday

Report Available

3 to 4 days

Specimen Retention Time

7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
RPMPM M. pneumoniae Macrolide Resist PCR 6483-2

 

Result ID Test Result Name Result LOINC Value
SRCMP Specimen source 31208-2
619928 M. pneumoniae Macrolide Resistance 6483-2

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.