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Test Code SHBG Sex Hormone Binding Globulin (SHBG)

Useful For

SHBG is the principle binding protein for testosterone and it primarily useful in combination with total testosterone to enable calculation of free testosterone.

Specimen Requirements

Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No Serum

Red Top Tube (RTT) or Serum Separator Tube (SST)

   0.5 mL 0.4 mL 0.3 mL

Collection/Processing Instructions

Separate serum from cells within 2 hours of collection.

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated 7 days
Frozen 2 months

Test Information

Grossly obese subjects will tend to have decreased levels; elevated levels are seen with patients receiving exogenous estrogen and those with hyperthyroidism.



The drug asfotase alfa (STRENSIQ), a recombinant form of alkaline phosphatase, is expected to interfere with in vitro diagnostic assays utilizing an alkaline phosphatase detection system. Test samples from patients taking asfotase alfa with a non-alkaline phosphatase methodology. 

Reference Range Information

Performing Location Reference Range
Marshfield Adult male: 10-61 nmol/L Adult female (non-pregnant): 19-125 nmol/L

Marshfield Labs Performing Department

Marshfield Labs Immunodiagnostics

Performing Information

Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield Monday through Friday 1 day Chemiluminescent Sandwich Immunoassay/ Immulite 2000

CPT Codes

CPT Modifier
(if needed)		 Quantity Description Comments
84270        

Outreach CPT Codes

CPT Modifier
(if needed)		 Quantity Description Comments
84270        

Ordering Applications

Ordering Application Description
Cerner Sex Hormone Binding Globulin (SHBG)