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Test Code T3-FREE Triiodothyronine, Free

Synonyms/Keywords

T-3, Free, T-3 Free, Free T3, FT3

Specimen Requirements

Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No Plasma/Serum Lithium Heparin Plasma Separator Tube (PST)

Lithium Heparin Green Top Tube (GTT), 

Serum Separator Tube (SST), 

Red Top Tube (RTT)

1 mL 0.5 mL 0.3 mL
  • GTT/RTT- physically separate plasma or serum from contact with cells as soon as possible of before transport. 
  • Separate from cells within 30 minutes to minimize uptake of corticosteroids by RBCs

Collection/Processing Instructions

Samples collected in a RTT must be removed from the cells within one hour for storage or transport. Samples collected in gel barrier tubes must be removed from the primary tube prior to transporting to Marshfield.

 

DO NOT send the primary collection tube.

Specimen Stability Information

Specimen Type Temperature Time

Plasma/Serum
 
Ambient 8 hours
Refrigerated 2 days
Frozen >2 days
 

 

  • Thaw samples no more than 3 times

Rejection Criteria

Gross Hemolysis Gross Icterus

Test Information

See Clinical Practice Guidelines: Thyroid Function Ordering Algorithm for more information

Interferences

Human Anti-Murine Antibodies (HAMA) may develop leading to test interference in patients receiving murine-based agents for chemotherapy or tumor-imaging studies.  

 

 

Reference Range Information

Performing Location Reference Range
Marshfield 0 to <1 year:  2.8 4.5 pg/mL
1 to <15 years:  2.6 4.0 pg/mL
Female 15 to <19 years:  2.3 3.5 pg/mL
Male 15 to <19 years:  2.5 3.7 pg/mL
Adult (>19 years):  2.5 3.9 pg/mL  

Marshfield Labs Performing Department

Marshfield Labs Chemistry

Performing Information

Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
 
Marshfield Monday through Sunday Less than 2 hours  Beckman Coulter DXI

CPT Codes

CPT Modifier
(if needed)
Quantity Description Comments
84481   1    

Outreach CPT Codes

CPT Modifier
(if needed)
Quantity Description Comments
84481   1    

Ordering Applications

Ordering Application Description
Cerner Triiodothyronine, Free