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HelpTest Code TBNK Immune Deficiency Cell markers, Blood
Useful For
For determining percentages and absolute counts of human helper/inducer (CD4) T-lymphocytes, suppressor/cytotoxic (CD8) T-lymphocytes, T-lymphocytes (CD3), natural killer lymphocytes (CD16+56), and B lymphocytes (CD19).
Synonyms/Keywords
Flow Cytometry, T&B Immunophenotyping, B & T Cells, Antibody Monitoring
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Whole blood | EDTA Lavender Top Tube (LTT) or EDTA Pink Top Tube (PTT) |
1 mL | 1 mL | 0.5 mL |
Collection/Processing Instructions
A CBC with differential is required within 4 hours of collection of specimen for this test. Submit results with the specimen for this test or include an order for a CBC with differential.
Specimen must arrive in Flow Cytometry within 72 hours of collection.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole blood | Room temperature | 72 hours |
Rejection Criteria
Frozen or refrigerated specimens
**Contact Flow Cytometry for suitability prior to rejection of any specimens.**
Test Information
Results reported include CD3 (T-cell total); T-cell absolute CD4 and CD8, including ratio; CD19 (B-cell total); and CD16+56 (Natural killer cells).
Absolute counts for this test are based on the CBC results provided at the time of testing, including lymphocyte percentage.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield | Age appropriate ranges printed on report |
Marshfield Labs Performing Department
Marshfield Labs Flow Cytometry
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday, Wednesday, and Friday | 1 day | Flow Cytometry |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86359 | T-cells | |||
| 86360 | Absolute CD4 and CD8 | |||
| 86357 | Natural killer cells | |||
| 86355 | B cells |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86359 | T-cells | |||
| 86360 | Absolute CD4 and CD8 | |||
| 86357 | Natural killer cells | |||
| 86355 | B cells |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | Immune Deficiency Cell Markers |