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HelpTest Code Tissue IHC, Surg Path Tissue Immunohistochemistry, Surgical Pathology
Useful For
Immunohistochemistry (IHC) and In Situ Hybridization (ISH) are techniques that use specific antibodies/probes to detect and bind to their specific antigens/nucleic acids in tissues/cells. In identifying specific molecules, their presence, or absence, distribution, and/or amount can help to diagnose and classify disease, normal, or benign processes. Used in conjunction with surgical pathology, abnormal cells characteristically found in particular cancerous tumors can be interpreted and classified, microorganisms identified, and prognostic and therapeutic options determined.
Synonyms/Keywords
Histology, Immunohistochemistry, IHC, Surgical Pathology, Paraffin Blocks
Specimen Requirements
| Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|
| Paraffin Embedded Tissue Block or Unstained Slide | Transport Container for Block and/or Slide Holders |
Acceptable Specimen Types
Paraffin-Embedded, Formalin Fixed Tissue Blocks, H&E Slide, and/or Unstained Air-Dried, Charged Slides
Collection/Processing Instructions
All (With or Without Interpretive Consult)
- List type of fixative, if other than NBF.
- Completed histology requisition form, with pertinent patient history and tissue source.
- One paraffin-embedded tissue/cell block and/or 3-4 micron, unstained (tissue sections placed on charged slides, then air-dried.
- H&E slide.
- Out Side Accounts - Surgical/Cytology Pathology Report
Interpretation of stains requires a consult.
Antibody validations were performed on 10% neutral buffered, formalin-fixed, paraffin embedded tissues/cell blocks. Many validations also included testing on alcoholic, zinc formalin fixed, paraffin embedded bone marrow specimens.
All blocks and slides will be returned to the requestor, including controls.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
|
Paraffin Tissue / Cell Block Fixed in 10% Neutral Buffered Formalin |
Room Temperature | |
| Unstained, Air-Dried, Charged Slides | Room Temperature | Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut fresh. |
Rejection Criteria
| Unstained Slides (No Charge on Slides) |
|---|
| Insufficient Data (Incomplete Requisition Form) |
Test Information
For List of Specific Stains see: Immunohistochemistry (IHC) Services
Blocks, H&E slides, Stained Slides, and their controls will be returned to sender upon test completion.
Interferences
Procedures have been validated on tissues fixed in 10% Neutral Buffered Formalin (NBF). Poorly fixed tissue, use of fixatives other than NBF, and/or decalcified tissue may cause unexpected or poor results.
Interpretations
With Consultation – See Surgical Pathology Report.
Classification
This test was developed and its performance characteristics determined by Marshfield Labs. It has not been cleared or approved by the US Food and Drug Administration. This test is used for clinical purposes. It should not be regarded as investigational or for research.
Marshfield Labs Performing Department
Marshfield Labs Histology
Performing Information
| Performing Location | Day(s) Test Performed | Methodology/Instrumentation |
|---|---|---|
| Marshfield | Monday - Friday | IHC & ISH Polymer-Based/Automated |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 88342T | IHC Stain Without Consultation & Interpretation | Each first stain | ||
| 88341T | IHC Stain Without Consultation & Interpretation | Each additional stain on the same specimen | ||
| 88342 | IHC Stain With Interpretation & Additional Consultation Charge | Each first stain | ||
| 88341 | IHC Stain With Interpretation & Additional Consultation Charge | Each additional stain on same specimen | ||
| 88365T | ISH Stain Without Consultation & Interpretation | Each first probe | ||
| 88364T | ISH Stain Without Consultation & Interpretation | Each additonal probe on the same specimen | ||
| 88365 | ISH Stain With Interpretation & Additional Consultation Charge | Each first probe | ||
| 88364 | ISH Stain With Interpretation & Additional Consultation Charge | Each additional probe on the same specimen |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 88342T | IHC Stain Without Consultation & Interpretation | Each first stain | ||
| 88341T | IHC Stain Without Consultation & Interpretation | Each additional stain on the same specimen | ||
| 88342 | IHC Stain With Interpretation & Additional Consultation Charge | Each first stain | ||
| 88341 | IHC Stain With Interpretation & Additional Consultation Charge | Each addtional stain on same specimen | ||
| 88365T | ISH Stain Without Consultation & Interpretation | Each first probe | ||
| 88364T | ISH Stain Without Consultation & Interpretation | Each additional probe on the same specimen | ||
| 88365 | ISH Stain With Interpretation & Additional Consultation Charge | Each first probe | ||
| 88364 | ISH Stain With Interpretation & Additional Consultation Charge | Each addtional probe on the same specimen |