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HelpTest Code UBKQNSO BK Virus DNA Detection and Quantification, Random, Urine
Additional Codes
Mayo Test Code: UBKQN
Specimen Required
Supplies: COBAS PCR - Urine Sample Kit (T903)
Container/Tube: cobas PCR urine tube
Specimen Volume: 4.3 mL
Collection Instructions:
1. Collect random urine into a sterile, plastic, preservative-free container.
2. Transfer 4.3 mL of urine into the cobas PCR urine sample tube using the disposable pipette provided within 24 hours of collection. The correct volume of urine has been added when the fluid level is between the black fill lines on the urine transport tube. Place the labels on the transport tube so the black fill lines are still visible for volume confirmation at Mayo Clinic Laboratories.
3. Transport and store urine specimen transport container at 2 to 30° C (refrigerate is preferred temperature).
Additional Information: cobas PCR media contains guanidine hydrochloride. Do not allow these tubes to come in direct contact with sodium hypochlorite (bleach) or other highly reactive reagents such as acids and bases. These mixtures can release a noxious gas.
Useful For
Detection and serial monitoring of BK virus (BKV)-associated nephropathy in kidney transplant recipients using random urine specimens
Detection and serial monitoring of BKV-associated hemorrhagic cystitis in organ transplant recipients
Method Name
Real-Time Polymerase Chain Reaction (RT-PCR)
Reporting Name
BKV DNA Detect/Quant, USpecimen Type
UrineSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Urine | Refrigerated (preferred) | 84 days | COBAS PCR URINE |
| Ambient | 84 days | COBAS PCR URINE |
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Cautions
On average, quantitative BK virus (BKV) DNA results in urine tested with this assay can be up to 3.5-fold (about 0.54 log IU/mL) higher than those generated from the previous laboratory-developed BKV DNA quantification assay performed at Mayo Clinic Laboratories, due to differences in the specimen extraction method and design in the amplification primers and probes for the viral target sequences.
A single "Undetected" test result does not necessarily rule out the presence BKV infection or reactivation. Serial measurement (eg, once weekly) of BKV DNA in urine or plasma is recommended to determine the BKV replication status in a given transplant recipient.
While unlikely to be present in urine specimens, vaginal lubricants, speculum jellies, creams, and gels containing carbomers may interfere with the test and should not be used during or prior to sample collection. Urogenital specimens from patients who have used carbomer-containing products such as Replens Long-Lasting Vaginal Moisturizer, RepHresh Odor Eliminating Vaginal Gel, and RepHresh Clean Balance or used metronidazole vaginal gel may generate invalid or false-negative results.
Day(s) Performed
Monday through Saturday
Report Available
1 to 3 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87799
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| UBKQN | BKV DNA Detect/Quant, U | 32285-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 614568 | BKV DNA Detect/Quant, U | 32285-9 |
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Microbiology Test Request (T244)
Reference Values
Undetected