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HelpTest Code VITB6 Vitamin B6 Pyridoxal 5-Phosphate (PLP), in Serum
Useful For
Determining vitamin B6 status and the overall success of a vitamin B6 supplementation progam.
Synonyms/Keywords
B6 (Vitamin), PALP (Pyridoxal 5-Phosphate), Pyridoxal 5-Phosphate (PALP), Pyridoxal Phosphate, Vitamin B6, PLP
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| Yes | Serum | Red Top Tube (RTT) | Heparin Green Top Tube (GTT) | 0.5 mL | 0.25 mL | 0.15 mL |
Collection/Processing Instructions
1) Fasting overnight (at least 9 hours) (infants, draw prior to next feeding)
2) Patient should not ingest vitamin supplements for 24 hours before specimen is drawn. 3) Foil wrap sample after collection and processing.
4) Remove serum/plasma from cells promptly after collection. Protect aliquot from light.
5) Use of gel separator tubes are discouraged, but will not be rejected so long as serum/plasma is removed from gel barrier within 12 hours of collection.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum/Plasma | Frozen |
At least 1 month |
| Refrigerate | up to 48 hours |
Rejection Criteria
EDTA Lavender Top Tube (LTT)
Serum Separator Tube (SST)/Plasma Separator Tube (PST) on gel >12 hours
Serum/plasma not protected from light >12 hours
Test Information
This test was developed and its performance characteristics determined by Marshfield Labs. It has not been cleared or approved by the US Food and Drug Administration. This test is used for clinical purposes. It should not be regarded as investigational or for research.
Vitamin B6 is a complex of 6 vitamers: pyridoxal, pyridoxol, pyridoxamine, and their 5'-phosphate esters. Due to its role as a cofactor in a number of enzymatic reactions, pyridoxal phosphate (PLP) has been determined to be the biologically active form of vitamin B6.
Vitamin B6 deficiency is uncommon and due to its function as a cofactor in many reactions of amino acid metabolism., it can have a wide range of symptoms including anemia, itchy rashes, scaly skin on the lips, cracks at the corners of the mouth, swollen tongue, depression, confusion, and a weak immune system. Infants who do not get enough vitamin B6 can become irritable, develop extremely sensitive hearing, or experience seizures.
Interpretations
Levels for fasting individuals falling in the range of 5 to 50 ug/L for pyridoxal-5’-phosphate (PLP) are indicative of adequate nutrition.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield | 5-50 ug/L |
Marshfield Labs Performing Department
Marshfield Labs Special Chemistry
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | 1 day/week: Tuesday | 2 - 4 hours | Liquid Chromatography-Tandem Mass Spectrometry (UPLC MS/MS) |
| Samples must be in performing department by 8am on day of testing for results the same day. | |||
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 84207 |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 84207 |
Ordering Applications
| Ordering Application | Description |
|---|---|
| COM | Vitamin B6, Pyridoxal-5-Phospyhate (PLP), in Serum |
| Cerner | Vitamin B6 |