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HelpTest Code VITD Vitamin D, 25-Hydroxy D2 and D3, Serum
Useful For
This is the first-line test for assessing Vitamin D status. Vitamin D is a fat-soluble vitamin that is involved in mineral homeostasis and plays an important role in calcium metabolism, parathyroid hormone release (PTH) and osteoblast formation. 25-hydroxyvitamin D levels are used to diagnose intestinal malabsorption, and vitamin D deficiency or intoxication. Additionally, 25-hydroxyvitamin D levels may be used to monitor therapeutic response in patients being treated for vitamin D-related disorders.
Synonyms/Keywords
Calcidiol, 25-hydroxy Vitamin D, 25-hydroxycholecalciferol, Vitamin D, Vitamin D2 + D3, Total Vitamin D, 25-OH Vitamin D
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Serum | Red Top Tube (RTT) | Lithium-heparin Plasma Separator (PST), EDTA Lavender Top (LTT), Serum Separator Tube (SST) Specimens in polypropylene aliquot tubes from Marshfield automation line are unacceptable and will be rejected from testing. |
0.5 mL Serum | 0.5 mL Serum | 0.3 mL Serum |
Collection/Processing Instructions
Collect specimen in preferred/acceptable containers as listed above in "Specimen Requirements".
Separate serum/plasma, then aliquot into "Serum Transfer Tube" (available through Marshfield Labs) or a clean plastic tube.
Refrigerate sample.
Further instructions for Marshfield specimen receiving/processing: Specimens aliquotted into polypropylene aliquot tubes from automation line are unacceptable and will be rejected from testing. Do not aliquot into automation line aliquot tubes!
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum/Plasma | Refrigerate | 2 weeks |
| Frozen | 1 month |
Test Components
25-Hydroxyvitamin D2, 25-Hydroxyvitamin D3 and Total 25-Hydroxyvitamin D
Test Information
This test was developed and its performance characteristics determined by Marshfield Labs. It has not been cleared or approved by the US Food and Drug Administration. This test is used for clinical purposes. It should not be regarded as investigational or for research.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield |
Specific reference ranges for 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 are not available
Reference range information is available for the total 25-hydroxyvitamin D and is as follows:
<12 ng/mL indicates severe deficiency with risk of rickets or osteomalacia
12 - 29 ng/mL mild to moderate deficiency with increased risk of osteoporosis
30 - 50 ng/mL optimum levels
51 - 80 ng/mL has increased risk of hypercalciuria
> 80 ng/mL toxicity possible.
Only the total 25-hydroxyvitamin D level should be considered when assessing 25-hydroxyvitamin D status. |
Marshfield Labs Performing Department
Marshfield Labs Special Chemistry
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield | Monday through Friday | 2-4 days | UPLC MS/MS - Waters Acquity UPLC, XEVO TQ-S Tandem Mass Spectrometry System |
| Samples are run/analyzed overnight; results are available next business day. | |||
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 82306 |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 82306 |
Ordering Applications
| Ordering Application | Description |
|---|---|
| Cerner | Vitamin D, 25-Hydroxy D2 and D3, Serum |
| COM | Vitamin D (25 Hydroxy) Total, D2, D3 |