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Test Code VWF:AG Von Willebrand Factor Antigen

Synonyms/Keywords

Factor VIII Related Antigen, FVIIIR:Ag

Specimen Requirements

Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
*Platelet Poor Plasma 3.2% Citrated Blue Top Tube (BTT)Citrated Blue Top Tube (BTT)   0.75 mL 0.75 mL 0.5 mL

Collection/Processing Instructions

One 0.75 mL citrated Blue Top Tube (BTT) plasma aliquot.  (If more than one coagulation test is ordered, a separate aliquot is needed for each test.)

  • Collect in Citrated Blue Top Tube (BTT)
  • Citrate anticoagulant must be adjusted for HCT >55%
  • Tube must be at least 90% full
  • Invert completely 3-4 times (without shaking) to mix


*See Instructions for: Preparation of Platelet Poor Plasma

Specimen Stability Information

Specimen Type Temperature Time
Plasma Frozen 6 months

Rejection Criteria

Clotted or Hemolyzed
Contamination with IV or Hickman Line fluids
Specimens that thaw during storage or transport

Test Information

Patients with blood group O have approximately 25% lower levels of von Willebrand factor and antigen. If the ABO blood group is not known on the patient, ABO blood typing is recommended to properly interpret the results of laboratory tests for von Willebrand's disease.


Contact Marshfield Labs 24 Hour Services at 800-222-5835, ext. 16383 with pre-operative requests.

Reference Range Information

Performing Location Reference Range
Marshfield 42-176 %

Marshfield Labs Performing Department

Marshfield Labs Hematology

Performing Information

Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield Monday and Thursday Less than 2 hours Immunoturbidimetric/ACL TOP550

CPT Codes

CPT Modifier
(if needed)
Quantity Description Comments
85246        

Outreach CPT Codes

CPT Modifier
(if needed)
Quantity Description Comments
85246        

Ordering Applications

Ordering Application Description
Centricity Von Willebrand Factor Ag
Cerner Von Willebrand Factor Antigen
COM Von Will Factor Antigen