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HelpTest Code HITAB Heparin Induced Thrombocytopenia Antibodies
Useful For
Diagnosing heparin-induced thrombocytopenia.
Synonyms/Keywords
Heparin Induced Antibody, HIT, PF4
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume (allows for 1 repeat) |
Pediatric Minimum Volume (no repeat) |
|---|---|---|---|---|---|---|
| No | Citrated Plasma | Citrated Blue Top Tube (BTT) | 3.0 mL | 1.0 mL |
Collection/Processing Instructions
Collect two citrated Blue Top Tubes (BTT) to make two 1.0 mL platelet poor plasma aliquots. (If other coagulation testing is ordered, separate aliquots are needed for each individual test.)
Citrate anticoagulant must be adjusted for HCT >55%
Tube must be at least 90% full
Invert completely 3-4 times (without shaking) to mix
*See Instructions for: Preparation of Platelet Poor Plasma
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma | Ambient | 4 hours |
| Frozen (-70° C) | 6 months |
Rejection Criteria
| Clotted or Hemolyzed | |
|---|---|
| Contamination with IV or Hickman Line Fluids | |
| Specimens that thaw during storage or transport |
Test Components
HIT antibody
Note: Testing will not be performed if patient has platelet counts ≥ 150,000. Testing will be paused, the sample will be held for 5 days, after which testing will be cancelled and credited. Please contact the lab for approval for testing of patients without thrombocytopenia.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| Marshfield |
Negative: This result has approximately 90-95% negative predictive value for excluding heparin-induced thrombocytopenia (HIT)
Positive: Heparin dependent antibodies are present. This result has a reasonably good correlation with the serotonin release assay (SRA). Serotonin Release Assay (SRA) has been ordered and will be performed by Versiti. Results should be available within 2-3 days. If the clinical situation warrants, initiating treatment for heparin-induced thrombocytopenia (HIT) is reasonable. |
Marshfield Labs Performing Department
Marshfield Labs Coagulation
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation |
|---|---|---|---|
| Marshfield |
Monday through Friday - twice per day Weekends/Holidays - as needed |
Less than 2 hours |
ACL TOP 550 |
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86022 | 1 | Heparin antibodies | ||
| 86022 | 1 | Serotonin, send-out to Versiti | if needed |
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86022 | 1 | Heparin antibodies | ||
| 86022 | 1 | Serotonin, send-out to Versiti | if needed |