Test Code BBABID Antibody Identification, RBC Antibodies
Useful For
Identifying unexpected red cell antibodies when the antibody screen is positive. This is important to provide compatible, antigen negative red cell products for transfusion.
Identifying antibody(ies) in prenatal patients to assist in determining the risk for Hemolytic Disease of the Fetus and Newborn (HDFN).
Synonyms/Keywords
Atypical Antibody Panel, Antibody ID, Red Cell Antibody, ABID
Specimen Requirements
| Fasting Required | Specimen Type | Preferred Container/Tube | Acceptable Container/Tube | Specimen Volume | Specimen Minimum Volume | Pediatric Minimum Volume |
|---|---|---|---|---|---|---|
| No | Whole blood | Two 6 mL EDTA Pink Top Tube (PTT) and 6 mL Red Top Tube (RTT) |
Two 3 mL EDTA Lavender Top Tubes (LTT)
OR
One 6 mL EDTA Pink Top Tube (PTT) |
6 mL | 3.0 mL | 3.0 mL |
Collection/Processing Instructions
Invert specimen at least 5-6 times in order to facilitate mixing and prevent clotting. Do not separate plasma from red cells.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole blood/serum | Refrigerate | <10 days old |
Rejection Criteria
| Serum separator tubes (SST) |
|---|
| Frozen |
| Hemolyzed |
Test Components
Antibody identification is not a provider orderable test; FOR INTERNAL USE ONLY. Non-Marshfield Clinic Health System Laboratories requesting antibody identification should order ABSC.
Testing may include the following components: ABSC, ABID, DAT
Test Information
For other laboratories solely requesting Antibody Identification for positive Antibody Screen results: Contact Transfusion Service with current test results and patient information at 800-222-5835 ext. 16262 before sending specimen. Complete a Request for Additional Serologic Testing at Marshfield Center form to Include patient diagnosis, history of transfusions, pregnancies and administration of Rh(D) Immune Globulin, if applicable. Send copies of serological work and include a copy of screen cell antigram.
Other Testing Performed: Tests may or may not be performed, at additional charge depending on the results and interpretation of the initial tests. Refer to CPT tables for additional testing codes and description.
The following common antibodies (not inclusive) are considered clinically significant and have been implicated in Hemolytic Disease of the Fetus and Newborn and/or hemolytic transfusion reactions: Anti-D, Anti-E, Anti-C, Anti-e, Anti-c, Anti-K, Anti-k, Anti-Fya, Anti-Fyb, Anti-Jka, Anti-Jkb, Anti-S, Anti-s, and IgG form of Anti-M. Antigen negative red cells should be selected for transfusion when antibodies are detected or patient has a history of the antibody.
Interferences
Fresh serum, fibrin or particulate matter in the patient specimen may cause anomalous results. EDTA plasma may minimize these interferences.
Reference Range Information
| Performing Location | Reference Range |
|---|---|
| All performing sites | No reference ranges are available. |
Marshfield Labs Performing Department
Marshfield Labs Transfusion Services
Performing Information
| Performing Location | Day(s) Test Performed | Analytical Time | Methodology/Instrumentation | |
|---|---|---|---|---|
| Marshfield | Monday through Sunday | typically 4 hours* | Solid Phase Red Cell Adherence PEG enhanced ID panel | |
| *Based on complexity of antibodies present, may take up to 24 hours. | ||||
CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86870 | ABID (additional antibody panels) | |||
| 86077 | PATH CONSULT | |||
| 86850 | ABSC | |||
| 86880 | DAT | |||
| 86905 | RBC antigen (patient or donor unit) | |||
| 86886 | Antibody titer | |||
| 86860 | Elution | |||
| 86978 | Cold adsorption* | |||
| 86978 | Warm adsorption* | |||
| 86971 | Ficin treatment | |||
| 86977 | Neutralization or acidification | |||
| 86920 86921 86922 | IAT crossmatch | |||
| *charge will be for quantity of adsorptions performed | ||||
Outreach CPT Codes
| CPT | Modifier (if needed) |
Quantity | Description | Comments |
|---|---|---|---|---|
| 86870 | ABID (additional antibody panels) | |||
| 86077 | PATH CONSULT | |||
| 86850 | ABSC | |||
| 86880 | DAT | |||
| 86905 | RBC antigen (patient or donor unit) | |||
| 86886 | Antibody titer | |||
| 86860 | Elution | |||
| 86978 | Cold adsorption* | |||
| 86978 | Warm adsorption* | |||
| 86971 | Ficin treatment | |||
| 86977 | Neutralization or acidification | |||
| 86920 86921 86922 | IAT crossmatch | |||
| *charge will be for quantity of adsorptions performed | ||||